Sukackas Justinas, Žilius Modestas, Šaltytė Gerda, Raudonė Lina
Department of Pharmacognosy, Lithuanian University of Health Sciences, Sukileliu av. 13, LT-50162 Kaunas, Lithuania.
Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu av. 13, LT-50162 Kaunas, Lithuania.
Antioxidants (Basel). 2025 Jul 7;14(7):831. doi: 10.3390/antiox14070831.
L. is a traditionally used medicinal plant rich in tannin compounds with antioxidant, anti-inflammatory, and antimicrobial activities. This study aimed to optimise the extraction of individual phenolic acids, flavonoids, and tannins from and maximise their antioxidant activity using response surface methodology (RSM). A central composite design was applied to evaluate the influence of acetone concentration, solvent ratio, and extraction time on the yield of total phenolics, total radical scavenging and reducing capacities, and individual compounds. Acetone concentration, solvent ratio, and extraction time were varied in a central composite design. The optimal conditions yielded high levels of agrimoniin (9.16 mg/g), total identified phenolics (33.61 mg/g), and strong antioxidant activity. These findings provide a scientific basis for standardising bioactive-rich extracts for nutraceutical and pharmaceutical applications.
L.是一种传统上使用的药用植物，富含具有抗氧化、抗炎和抗菌活性的单宁化合物。本研究旨在优化从该植物中提取单个酚酸、黄酮类化合物和单宁，并使用响应面法（RSM）最大化它们的抗氧化活性。采用中心复合设计来评估丙酮浓度、溶剂比例和提取时间对总酚产量、总自由基清除能力和还原能力以及单个化合物的影响。在中心复合设计中改变丙酮浓度、溶剂比例和提取时间。最佳条件下可获得高水平的仙鹤草素（9.16毫克/克）、总鉴定酚类（33.61毫克/克）以及较强的抗氧化活性。这些发现为标准化用于营养保健品和制药应用的富含生物活性提取物提供了科学依据。
