College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
College of Agriculture and Bioengineering, Heze University, Heze, 274000, Shandong, China.
BMC Vet Res. 2023 Nov 21;19(1):242. doi: 10.1186/s12917-023-03806-x.
Salmonella Enteritidis is a zoonotic pathogen and poses a substantial risk to human health, as well as significant financial losses to the livestock and poultry industries. It is currently urgent to identify alternatives to antibiotic treatment.
In this study, we explored the influence of Puerarin on the immunological response, intestinal flora, serum metabolome, and growth performance of chicks infected with Salmonella Enteritidis. Chicks were weighed at specific time points and the average daily gain (ADG) was calculated. Serum, intestinal, and cecal content samples were collected on days 10 and 17. The results showed that 100 mg/kg of Puerarin significantly suppressed inflammation and enhanced immune function. Metabolomic analysis showed significant differences in serum metabolites after Puerarin treatment and suggested that Puerarin may regulate abnormal amino acid and lipid metabolism after Salmonella Enteritidis infection through the autophagic and ABC transporter pathways. In addition, Puerarin suppressed Salmonella Enteritidis-induced intestinal flora dysbiosis through modulation of the microbial community structures (increased Lactobacillus, Faecalibacterium, and Subdoligranulum), as demonstrated by 16S rRNA analysis.
In conclusion, Puerarin can improve growth performance in chicks, suppress the inflammatory response in vivo, enhance immunity, and regulate lipid and amino acid metabolism and the intestinal flora.
肠炎沙门氏菌是一种人畜共患病原体，对人类健康构成重大威胁，同时也给畜牧业带来重大经济损失。目前迫切需要寻找抗生素治疗的替代品。
本研究探讨了葛根素对感染肠炎沙门氏菌的雏鸡免疫反应、肠道菌群、血清代谢组和生长性能的影响。在特定时间点对雏鸡进行称重，并计算平均日增重（ADG）。在第 10 天和第 17 天收集血清、肠道和盲肠内容物样本。结果表明，100mg/kg 的葛根素可显著抑制炎症反应，增强免疫功能。代谢组学分析表明，葛根素处理后血清代谢物存在显著差异，提示葛根素可能通过自噬和 ABC 转运体途径调节肠炎沙门氏菌感染后的异常氨基酸和脂质代谢。此外，通过 16S rRNA 分析，葛根素通过调节微生物群落结构（增加乳酸杆菌、粪杆菌和副拟杆菌）抑制肠炎沙门氏菌诱导的肠道菌群失调。
综上所述，葛根素可以提高雏鸡的生长性能，抑制体内炎症反应，增强免疫力，调节脂质和氨基酸代谢以及肠道菌群。
