Identification of Molecular Subtypes of B-Cell Acute Lymphoblastic Leukemia in Mexican Children by Whole-Transcriptome Analysis.

B-lineage acute lymphoblastic leukemia (B-ALL) is classified into more than 20 molecular subtypes, and next-generation sequencing has facilitated the identification of these with high sensitivity. Bulk RNA-seq analysis of bone marrow was realized to identify molecular subtypes in Mexican pediatric patients with B-ALL. High hyperdiploidy (27.3%) was the most frequent molecular subtype, followed by (13.6%), (9.1%), (9.1%), (9.1%), (9.1%), (4.5%), Ph (4.5%), (4.5%), and (4.5%), with one patient presenting both the and low hypodiploidy subtypes (4.5%). The genes , and harbor deleterious missense variants across different B-ALL molecular subtypes. The subtype exhibited mutations in , , , , , and with overexpression of the gene. The subtype showed mutually exclusive missense variants in the gene. Here, we have demonstrated the importance of using RNA-seq to facilitate the differential diagnosis of B-ALL with successful detection of gene fusions and mutations. This will aid both patient risk stratification and precision medicine.