Derakhshan Safoura, Farhadifar Fariba, Roshani Daem, Ahmadi Amjad, Haghi Fakhri
Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Zoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Gastroenterol Hepatol Bed Bench. 2019 Fall;12(4):348-357.
This article aimed to analyze the diarrheagenic potential of isolated from urinary tract infection (UTI) and to recognize the presence of antibiotic resistance genes.
The marked genome plasticity of has allowed the emergence of resistant pathogenic strains displaying an unusual arrangement of genes.
In this cross-sectional study, 110 were isolated from patients with the symptoms of UTI in Sanandaj, west of Iran between July and September - 2015. The isolates were examined by the disk diffusion method for antibiotic susceptibility test and by polymerase chain reaction for the presence of genes characteristic of diarrheagenic (DEC), Uropathogenic () virulence genes, extended-spectrum β-lactamase and plasmid-mediated quinolone resistance determinants, , , and .
The most and the least effective antibiotics were nitrofurantoin and cefotaxime (96.4% and 27.3% sensitivity, respectively). Of the 110 UTI isolates, 57.3% carried diarrheagenic genes. The bundle-forming pilus was the most prevalent diarrheagenic gene (39.1%). The most commonly detected DEC pathotype was enterotoxigenic (ETEC, 12.7%). All the pathotypes carried the and . The UPEC hemolysin and adhesin genes were mainly detected among ETEC isolates.
Our results indicated the presence of resistant diarrheagenic pathotypes in UTI-associated . Such isolates may have the capacity of causing both extraintestinal and intestinal infections. Based on our knowledge, this is the first report of the presence of in ETEC from urine.
本文旨在分析从尿路感染（UTI）中分离出的[具体细菌名称未给出]的致腹泻潜力，并识别抗生素耐药基因的存在情况。
[具体细菌名称未给出]显著的基因组可塑性使得具有异常基因排列的耐药致病菌株得以出现。
在这项横断面研究中，于2015年7月至9月期间从伊朗西部萨南达季有UTI症状的患者中分离出110株[具体细菌名称未给出]。采用纸片扩散法对分离株进行抗生素敏感性试验，并通过聚合酶链反应检测是否存在致腹泻[具体细菌名称未给出]（DEC）的特征基因、尿路致病性[具体细菌名称未给出]（UPEC）毒力基因、超广谱β-内酰胺酶（ESBL）以及质粒介导的喹诺酮耐药决定子、[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]。
最有效和最无效的抗生素分别是呋喃妥因和头孢噻肟（敏感性分别为96.4%和27.3%）。在110株UTI分离株中，57.3%携带致腹泻基因。束状菌毛基因是最常见的致腹泻基因（39.1%）。最常检测到的DEC致病型是产肠毒素[具体细菌名称未给出]（ETEC，12. + 7%）。所有致病型均携带[具体基因名称未给出]和[具体基因名称未给出]。UPEC溶血素和黏附素基因主要在ETEC分离株中检测到。
我们的结果表明在与UTI相关的[具体细菌名称未给出]中存在耐药性致腹泻致病型。此类分离株可能具有引起肠外和肠道感染的能力。据我们所知，这是首次关于尿液中ETEC存在[具体细菌名称未给出]的报告。
