Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran.
BMC Vet Res. 2021 Mar 25;17(1):131. doi: 10.1186/s12917-021-02832-x.
Transmission of antimicrobial resistant and virulent Escherichia coli (E. coli) from animal to human has been considered as a public health concern. This study aimed to determine the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. In this research, the genes related to six main pathotypes of E. coli were screened. Also, genotypic and phenotypic antimicrobial resistance against commonly used antibiotics were studied, then phylo-grouping was performed on all the isolates.
Out of 65 analyzed isolates, 29.23 % (n = 19) were determined as STEC and 6.15 % (n = 4) as potential EPEC. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid (46.2 %) and ceftriaxone (38.5 %). bla was the most detected resistance gene (98.4 %) among the isolates and 26.15 % of the E. coli isolates were determined as multi-drug resistant (MDR). Three phylo-types including B1 (76.92 %), A (13.85 %) and D (3.08 %) were detected among the isolates.
Due to the close interaction of horses and humans, these findings would place emphasis on the pathogenic and zoonotic potential of the equine strains and may help to design antimicrobial resistance stewardship programs to control the dissemination of virulent and multi-drug resistant E. coli strains in the community.
动物源性耐药性和毒力大肠杆菌（E. coli）向人类传播一直被认为是公共卫生关注的问题。本研究旨在确定伊朗健康骑乘马中腹泻性大肠杆菌和抗生素耐药性的系统发育背景和流行情况。在这项研究中，筛选了与大肠杆菌六种主要血清型相关的基因。此外，还研究了针对常用抗生素的基因型和表型抗生素耐药性，然后对所有分离株进行了系统发育分组。
在分析的 65 株分离株中，29.23%（n=19）被确定为 STEC，6.15%（n=4）为潜在的 EPEC。最常见的抗生素耐药表型是对阿莫西林/克拉维酸（46.2%）和头孢曲松（38.5%）。bla 是分离株中检测到的最常见耐药基因（98.4%），26.15%的大肠杆菌分离株被确定为多药耐药（MDR）。在分离株中检测到三种系统发育型，包括 B1（76.92%）、A（13.85%）和 D（3.08%）。
由于马与人的密切互动，这些发现将强调马源菌株的致病性和人畜共患病潜力，并可能有助于设计抗生素耐药性管理计划，以控制社区中毒力和多药耐药大肠杆菌菌株的传播。
