Yan Danxi, Wen Shuyi, Chen Mingxia, Huang Jinlan, Zhang Guihao, Li Renkai, Lu Jiamin, Yao Zhongxuan, Gao Fei, You Jieshu
School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China.
College of Pharmacy, Shenzhen Technology University, Shenzhen 518118, China.
Curr Issues Mol Biol. 2025 Jul 11;47(7):541. doi: 10.3390/cimb47070541.
According to our research, the flowers from 'Tomentosa' contain rich biologically active essential oil components, but the chemical components and relative pharmacological properties have not been systematically studied. Therefore, the study aimed to identify the essential oil components by GC-MS/MS and explore the pharmacological activity and mechanism of these essential oil components by a network pharmacology approach. Finally, GC-MS/MS analysis identified 43 essential oil components, which corresponded to 739 potential targets. GO analysis results showed that 12, 18, and 12 entries were related to biological processes, cellular components, and molecular functions, respectively. A total of 120 pathways were obtained based on KEGG analysis, of which the most important was the adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway. The "active component-target-disease" network further demonstrated these essential oil components' potential efficacy against pain, tumors, neuropsychiatric diseases, eye diseases, and respiratory diseases, which were highly related to PPARA, GABRA1, PTGS2, and SLC6A2. Experimental validation confirmed that β-caryophyllene, a major constituent, dose-dependently inhibited the proliferation of HT29 and MCF-7 cells (0-320 μM). This study provides a reliable basis for elucidating the pharmacological activity of the essential oil components and related mechanisms, which is beneficial to the comprehensive utilization and development of 'Tomentosa'.
根据我们的研究，“绒毛番荔枝”的花含有丰富的具有生物活性的精油成分，但化学成分和相关药理特性尚未得到系统研究。因此，本研究旨在通过气相色谱-串联质谱法（GC-MS/MS）鉴定精油成分，并通过网络药理学方法探索这些精油成分的药理活性及作用机制。最终，GC-MS/MS分析鉴定出43种精油成分，对应739个潜在靶点。基因本体（GO）分析结果显示，分别有12、18和12个条目与生物过程、细胞成分和分子功能相关。基于京都基因与基因组百科全书（KEGG）分析共获得120条通路，其中最重要的是抑制腺苷酸环化酶的G蛋白偶联型乙酰胆碱受体信号通路。“活性成分-靶点-疾病”网络进一步证明了这些精油成分对疼痛、肿瘤、神经精神疾病、眼部疾病和呼吸系统疾病具有潜在疗效，这些疾病与过氧化物酶体增殖物激活受体α（PPARA）、γ-氨基丁酸A型受体α1亚基（GABRA1）、环氧化酶-2（PTGS2）和溶质载体家族6成员2（SLC6A2）高度相关。实验验证证实，主要成分β-石竹烯可剂量依赖性抑制HT29和MCF-7细胞的增殖（0-320μM）。本研究为阐明精油成分的药理活性及相关机制提供了可靠依据，有利于“绒毛番荔枝”的综合利用与开发。
