Talbot P
Am J Anat. 1985 Nov;174(3):331-46. doi: 10.1002/aja.1001740312.
Literature on the interactions between eutherian gametes is reviewed. The oocyte cumulus complex of the female is surrounded by a zona pellucida, corona radiata, and cumulus layer. Sperm undergo an acrosome reaction before penetrating the zona pellucida. The morphological consequences of this reaction and its possible role(s) in penetration of the oocyte cumulus complex are considered. The acrosomal enzyme, hyaluronidase, has been thought to aid sperm in penetrating the cumulus layer and corona radiata. Several recent investigations, including one that shows that motile cells lacking hyaluronidase can penetrate to the zona surface, do not support this idea. Other possible roles of this enzyme in fertilization are discussed. The development of in vitro fertilization systems that employ physiological numbers (1-100) of sperm will be valuable in studying the mechanisms used by sperm to penetrate the oocyte cumulus complex.
本文综述了有关真兽亚纲配子间相互作用的文献。雌性的卵母细胞-卵丘复合体被透明带、放射冠和卵丘层所包围。精子在穿透透明带之前会发生顶体反应。本文考虑了该反应的形态学后果及其在穿透卵母细胞-卵丘复合体过程中可能发挥的作用。顶体酶——透明质酸酶，曾被认为有助于精子穿透卵丘层和放射冠。最近的几项研究，包括一项表明缺乏透明质酸酶的活动细胞能够穿透至透明带表面的研究，并不支持这一观点。本文还讨论了该酶在受精过程中的其他可能作用。采用生理数量（1-100个）精子的体外受精系统的开发，对于研究精子穿透卵母细胞-卵丘复合体所使用的机制将具有重要价值。
