Neiders M E, Morse H C, Lawton A R, Asofsky R
J Immunol. 1977 Aug;119(2):457-64.
SAMM 368, a plasmacytoma which produces IgA-kappa and IgG2b-kappa was established in vitro from primary explants or after animal passage. The 9 lines that were established produced both paraproteins. The production of both immunoglobulins by single cells was demonstrated by immunofluorescent staining and cloning. Continuous culture of 3 parent lines for 18 months and 11 cloned lines for periods from 5 to 7 months demonstrated that double production is a stable characteristic of this plasmacytoma. Two single paraprotein-producing varients (IgG2b or IgA) were derived when cells were cultured in the presence of Fungizone. Chromosomal analysis of SAMM 368 indicates that this double producing tumor has a modal number similar to those observed in myelomas producing a single immunoglobulin class.
SAMM 368是一种产生IgA-κ和IgG2b-κ的浆细胞瘤，它是从原代外植体或经动物传代后在体外建立的。所建立的9个细胞系均产生两种副蛋白。通过免疫荧光染色和克隆证明单个细胞可产生两种免疫球蛋白。对3个亲代细胞系进行18个月的连续培养，对11个克隆细胞系进行5至7个月的培养，结果表明双蛋白产生是这种浆细胞瘤的一个稳定特征。当细胞在两性霉素B存在的情况下培养时，可产生两种产生单一副蛋白的变体（IgG2b或IgA）。对SAMM 368的染色体分析表明，这种双蛋白产生的肿瘤的众数与产生单一免疫球蛋白类别的骨髓瘤中观察到的相似。
