Yang Quan, Tan Shimeng, Niu Anqi, Liu Junang, Zhou Guoying
Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, China.
Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha 410004, China.
Microorganisms. 2025 Jun 23;13(7):1454. doi: 10.3390/microorganisms13071454.
The climate-driven acceleration of forest disease outbreaks has intensified the demand for sustainable biocontrol strategies. In this study, we evaluated the effects of the endophytic bacterium csuftcsp75 on soil properties, microbial communities, and functional metabolism in soils affected by shoot blight. Soil physicochemical analysis, carbon substrate utilization profiling (AWCD), and diversity indices (the Shannon, Simpson, and McIntosh indices) were integrated to assess the microbial responses under different inoculation treatments. The csuftcsp75 treatment significantly improved soil nutrient availability-especially available phosphorus and potassium-and was associated with enhanced microbial metabolic activity and sustained functional diversity. Principal component analysis and correlation mapping revealed strong associations between labile nutrients and microbial responses. Comparative analysis showed that csuftcsp75 promoted a balanced and metabolically rich microbial community, while less compatible strains exhibited transient or unstable effects. These findings support a dual-pathway model wherein nutrient-driven metabolic activation and ecological integration jointly determine biocontrol efficacy. This study highlights the importance of matching microbial inoculants with local soil environments to optimize functional outcomes. This work provides a theoretical basis for applying endophytic in forest disease management and contributes to the development of ecologically coherent biocontrol strategies.
气候驱动的森林病害爆发加速，增加了对可持续生物防治策略的需求。在本研究中，我们评估了内生细菌csuftcsp75对受枝枯病影响土壤的土壤性质、微生物群落和功能代谢的影响。综合土壤理化分析、碳底物利用谱（AWCD）和多样性指数（香农指数、辛普森指数和麦金托什指数）来评估不同接种处理下的微生物反应。csuftcsp75处理显著提高了土壤养分有效性，尤其是有效磷和钾，并与增强的微生物代谢活性和持续的功能多样性相关。主成分分析和相关图谱显示不稳定养分与微生物反应之间存在强关联。比较分析表明，csuftcsp75促进了一个平衡且代谢丰富的微生物群落，而相容性较差的菌株表现出短暂或不稳定的效果。这些发现支持了一个双途径模型，其中养分驱动的代谢激活和生态整合共同决定生物防治效果。本研究强调了使微生物接种剂与当地土壤环境相匹配以优化功能结果的重要性。这项工作为内生菌在森林病害管理中的应用提供了理论基础，并有助于制定生态连贯的生物防治策略。
