Institute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
College of Life Sciences, Yangtze University, Jingzhou, 434025, China.
Curr Microbiol. 2024 Jun 19;81(8):228. doi: 10.1007/s00284-024-03752-3.
Soil nutrient deficiency has become a key factor limiting crop growth. Plant growth-promoting rhizobacteria (PGPR) are vital in resisting abiotic stress. In this study, we investigated the effects of inoculation with Bacillus amyloliquefaciens JB20221020 on the physiology, biochemistry, rhizosphere microorganisms, and metabolism of lettuce under nutrient stress. Pot experiments showed that inoculation with B. amyloliquefaciens JB20221020 significantly promoted lettuce growth under nutrient deficiency. At the same time, the activities of the antioxidant enzymes superoxide dismutase, peroxidase, and catalase and the content of proline increased, and the content of Malondialdehyde decreased in the lettuce inoculated with B. amyloliquefaciens JB20221020. Inoculation with B. amyloliquefaciens JB20221020 altered the microbial community of the rhizosphere and increased the relative abundances of Myxococcales, Deltaproteobacteria, Proteobacteria, Devosia, and Verrucomicrobia. Inoculation also altered the rhizosphere metabolism under nutrient deficiency. The folate metabolism pathway was significantly enriched in the Kyoto Encyclopedia of Genes and Genomes enrichment analysis. This study explored the interaction between plants and microorganisms under nutrient deficiency, further explained the critical role of rhizosphere microorganisms in the process of plant nutrient stress, and provided a theoretical basis for the use of microorganisms to improve plant resistance.
土壤养分缺乏已成为限制作物生长的关键因素。植物促生根际细菌（PGPR）在抵抗非生物胁迫方面至关重要。在这项研究中，我们研究了在养分胁迫下接种解淀粉芽孢杆菌 JB20221020 对生菜生理、生化、根际微生物和代谢的影响。盆栽实验表明，接种解淀粉芽孢杆菌 JB20221020 显著促进了养分缺乏下生菜的生长。同时，接种解淀粉芽孢杆菌 JB20221020 后，生菜中超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性以及脯氨酸的含量增加，丙二醛的含量降低。接种解淀粉芽孢杆菌 JB20221020 改变了根际微生物群落，增加了粘球菌目、δ变形菌纲、变形菌门、Devosia 和疣微菌门的相对丰度。接种还改变了养分缺乏下的根际代谢。在京都基因与基因组百科全书富集分析中，叶酸代谢途径显著富集。本研究探讨了养分缺乏下植物与微生物之间的相互作用，进一步解释了根际微生物在植物养分胁迫过程中的关键作用，为利用微生物提高植物抗性提供了理论依据。
