Rintala Helena, Röhl Oliver, Tegelberg Pinja, Meklin Teija
Labroc Oy Member of GBA Group, P.O. Box 1199, 70211 Kuopio, Finland.
GBA Gesellschaft für Bioanalytik mbH, Schelsenweg 24a, 41238 Mönchengladbach, Germany.
Microorganisms. 2025 Jul 1;13(7):1551. doi: 10.3390/microorganisms13071551.
According to several reports, 10-50% of buildings in Europe and worldwide suffer from moisture problems, which can lead to microbial growth in building materials. Unrepaired moisture and microbial damage can lead to the degradation of building structures and reduce visual appeal, resulting in economic losses; they can also result in adverse health effects for the building's occupants. Consequently, robust and reliable methods for the detection of abnormal microbiological conditions in buildings are needed, alongside skilled technical investigations, to plan appropriate renovation actions. In this work, 964 building material samples, which were obtained as part of routine building investigations in two countries, were analyzed for their fungal content using the qPCR method. Cultivation analysis was performed using the same samples, according to corresponding national guidelines. In a sample subset, the total cell counts after staining with acridine orange were determined. The microbial concentrations obtained with all three methods correlated well. Threshold values for the qPCR results were determined using cultivation as a reference method for both countries separately, with similar values obtained for both datasets. Hence, qPCR has great potential to become a standard method of detecting microbes in indoor environments.
根据几份报告，欧洲及全球10%至50%的建筑物存在潮湿问题，这可能导致建筑材料中微生物滋生。未修复的潮湿和微生物损害会导致建筑结构退化并降低视觉吸引力，从而造成经济损失；它们还可能对建筑物居住者的健康产生不利影响。因此，除了进行专业的技术调查外，还需要强大且可靠的方法来检测建筑物中的异常微生物状况，以便规划适当的翻新行动。在这项工作中，作为两个国家常规建筑调查的一部分获取的964个建筑材料样本，采用定量聚合酶链反应（qPCR）方法分析其真菌含量。根据相应的国家指南，对相同样本进行培养分析。在一个样本子集中，测定了用吖啶橙染色后的总细胞数。用这三种方法获得的微生物浓度相关性良好。分别以培养法作为参考方法，为两个国家确定了qPCR结果的阈值，两个数据集得到了相似的值。因此，qPCR极有可能成为检测室内环境中微生物的标准方法。
