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重新审视不同噬菌体类型菌株的基因组流行病学揭示了疫情期间有限的时空传播。

Revisiting the Genomic Epidemiology of Distinct Phage-Type Strains Reveals Restricted Spatiotemporal Dissemination During an Epidemic.

作者信息

Jiang Yu, Zhao Wenxuan, Yang Xiaorong, Fan Fenxia, Li Zhenpeng, Pang Bo, Kan Biao

机构信息

National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

National Institute of Environment Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China.

出版信息

Microorganisms. 2025 Jul 5;13(7):1585. doi: 10.3390/microorganisms13071585.

Abstract

The El Tor biotype of caused the seventh cholera pandemic (7CP). Although variants of this biotype frequently emerge, studies on their microevolution and spatiotemporal transmission in epidemics caused by a single clone are limited. During the cholera outbreak in Sichuan Province, China, in the 1990s, strains belonging to phage type 6 (PT6) but resistant to typing phage VP5 due to a deletion mutation in , which is the gene associated with the VP5 receptor were identified. In this study, we analyzed PT6 strains using genome sequencing to reveal the genomic and transmission characteristics of such a transient phage type in China's cholera epidemic history. The findings revealed that the PT6 strains formed an independent clone during the four-year epidemic and emerged in wave 2. Most of them carried multiple CTXΦ genome copies on chromosome 2 (Chr. 2) and two copies each of RS1 and RS1-4** on chromosome 1 (Chr. 1). Frequent cross-regional transmission and local outbreaks within Sichuan Province, China, were revealed for this clone. A variety of spontaneous mutations in the gene, conferring resistance to the VP5 phage, were observed under VP5 infection pressure, showing the incident mutation of OmpW for the survival adaptation of to phage pressure. Therefore, this genomic epidemiological revisit of these distinct phage-resistant phenotype strains reveals their clonal genetic structure, improves our understanding of the spread of by tracking their variation, and assists in epidemic source tracing and disease control.

摘要

埃尔托生物型霍乱弧菌引发了第七次霍乱大流行(7CP)。尽管该生物型的变种频繁出现,但关于它们在由单一克隆引发的疫情中的微观进化和时空传播的研究却很有限。在20世纪90年代中国四川省的霍乱疫情期间,鉴定出了属于噬菌体分型6型(PT6)但因与VP5受体相关的基因发生缺失突变而对分型噬菌体VP5耐药的菌株。在本研究中,我们使用全基因组测序分析PT6菌株,以揭示这种在中国霍乱流行史上短暂出现的噬菌体分型的基因组和传播特征。研究结果表明,PT6菌株在为期四年的疫情期间形成了一个独立的克隆,并在第二波疫情中出现。它们中的大多数在2号染色体(Chr. 2)上携带多个CTXΦ基因组拷贝,在1号染色体(Chr. 1)上分别携带两个RS1和RS1-4**拷贝。该克隆在中国四川省内呈现频繁的跨区域传播和局部暴发。在VP5感染压力下,观察到了基因中多种赋予对VP5噬菌体耐药性 的自发突变,显示出OmpW为适应噬菌体压力而发生的偶发突变。因此,对这些具有独特噬菌体耐药表型菌株的基因组流行病学再研究揭示了它们的克隆遗传结构,通过追踪其变异提高了我们对霍乱弧菌传播的理解,并有助于疫情溯源和疾病控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e2/12300545/ba39841dd8bf/microorganisms-13-01585-g001.jpg

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