Yaguchi Erika, Komiyama Yuske, Inami Shu, Shibasaki Ikuko, Shintani Tomoaki, Shiraishi Ryo, Hyodo Toshiki, Shiba Hideki, Hamaguchi Shinsuke, Fukuda Hirotsugu, Toyoda Shigeru, Fukumoto Chonji, Izumi Sayaka, Wakui Takahiro, Kawamata Hitoshi
Department of Oral and Maxillofacial Surgery, Dokkyo Medical University School of Medicine, 880 Kitakobayashi, Shimotsuga, Mibu 321-0293, Tochigi, Japan.
Department of Cardiovascular Medicine, Dokkyo Medical University School of Medicine, 880 Kitakoba Yashi, Shimotsuga, Mibu 321-0293, Tochigi, Japan.
Microorganisms. 2025 Jul 16;13(7):1677. doi: 10.3390/microorganisms13071677.
The involvement of oral bacteria in the pathogenesis of distant organs, such as the heart, lungs, brain, liver, and intestine, has been shown. We analyzed the distribution of bacterial species in the resected aortic valve by 16S rRNA metagenomic analysis and directly compared their gene sequences with those in the oral cavity. Thirty-two patients with aortic stenosis or aortic regurgitation who underwent aortic valve replacement were enrolled in this study. Antibody titer against periodontal pathogenic bacteria in the patient's serum was analyzed. The genetic background and distribution of bacterial species on subgingival plaque, the dorsal surface of the tongue, and the resected aortic valve were analyzed. Patients with aortic valve disease were shown to have more severe periodontal disease by the detection of antibodies against Socransky's red-complex bacteria of periodontitis. Bacterial DNA was detected in the aortic valves of 12 out of 32 patients. The genomic sequences of the V3-V4 region of the 16S rRNA in some bacteria isolated from the aortic valves of six patients who underwent metagenomic analysis were identical to those found in the oral cavity. The findings indicate that bacteria detected in the aortic valve may be introduced through oral dysbiosis, a condition characterized by an imbalance in the oral microbiota that increases the risk of periodontal disease and dental caries. Oral dysbiosis and the resulting potential bacteremia are associated with the pathogenesis of aortic valve diseases.
口腔细菌在诸如心脏、肺、脑、肝和肠道等远处器官发病机制中的作用已得到证实。我们通过16S rRNA宏基因组分析来分析切除的主动脉瓣中细菌种类的分布，并将其基因序列与口腔中的基因序列进行直接比较。本研究纳入了32例接受主动脉瓣置换术的主动脉瓣狭窄或主动脉瓣关闭不全患者。分析了患者血清中针对牙周病原菌的抗体滴度。分析了龈下菌斑、舌背和切除的主动脉瓣上细菌种类的遗传背景和分布。通过检测针对牙周炎Socransky红色复合体细菌的抗体，发现患有主动脉瓣疾病的患者患有更严重的牙周疾病。在32例患者中的12例主动脉瓣中检测到细菌DNA。对6例接受宏基因组分析的患者的主动脉瓣中分离出的一些细菌的16S rRNA的V3-V4区域的基因组序列与口腔中发现的序列相同。这些发现表明，在主动脉瓣中检测到的细菌可能是通过口腔生态失调引入的，口腔生态失调是一种以口腔微生物群失衡为特征的状况，会增加患牙周病和龋齿的风险。口腔生态失调及由此产生的潜在菌血症与主动脉瓣疾病的发病机制有关。
