大鼠3D打印诱导装置（RAPID-3D）：一种用于在大鼠中进行均匀且可重复的烫伤诱导的3D打印装置，并进行了组织学和微血管验证。

Rat 3D Printed Induction Device (RAPID-3D): A 3D-Printed Device for Uniform and Reproducible Scald Burn Induction in Rats with Histological and Microvascular Validation.

作者信息

Roșca Oana-Janina, Nistor Alexandru, Brandabur Călin, Heredea Rodica Elena, Hoinoiu Bogan, Șoica Codruța

机构信息

Discipline of Clinical Practical Skills, Department I Nursing, Faculty of Medicine, Victor Babeș University of Medicine and Pharmacy, 300041 Timișoara, Romania.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Victor Babeș University of Medicine and Pharmacy, 300041 Timișoara, Romania.

出版信息

Biology (Basel). 2025 Apr 7;14(4):378. doi: 10.3390/biology14040378.

DOI:10.3390/biology14040378

PMID:40282243

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12025246/

Abstract

BACKGROUND

Scald burns are common thermal injuries in clinical settings, yet existing animal models lack standardization in burn size, exposure time, and severity control. Traditional burn induction methods, such as manual immersion or heated metal contact, suffer from high variability, limited reproducibility, and are operator-dependent, reducing their translational relevance. This study presents RAPID-3D (rat printed induction device-3D), a novel 3D-printed system designed to induce uniform and reproducible scald burns in a rat model, ensuring precise exposure control and minimal variability.

METHODS

RAPID-3D features four burn exposure windows (10 × 20 mm each, 10 mm spacing), allowing for controlled boiling water (100 °C, 8 s) exposure while immobilizing the anesthetized rat's dorsum. N = 10 female Wistar rats were subjected to eight controlled burns per animal. Internal unburned control areas were used in each rat for intra-animal comparison. Burn evolution was assessed using digital planimetry, histological evaluation, and real-time microvascular perfusion analysis via laser Doppler line scanning (LDLS) at 1 h, which was repeated on day 4, 9 and 21 post-burn.

RESULTS

RAPID-3D generated highly consistent burn sizes (198 ± 3.54 mm) across all rats, with low inter-animal variability. Histological analysis confirmed full-thickness epidermal necrosis and deep partial-thickness dermal damage (600-900 µm depth). Microvascular Trends: Perfusion dropped immediately post-burn, remained low at day 4, and gradually increased from day 9 onward, suggesting progressive neovascularization and vascular remodeling. RAPID-3D provides a standardized, reproducible, and clinically relevant scald burn model, eliminates operator-induced variability, enhances experimental consistency, and offers strong translational relevance for burn treatment development and wound healing research.

摘要

背景

烫伤是临床常见的热损伤，但现有的动物模型在烧伤面积、暴露时间和严重程度控制方面缺乏标准化。传统的烧伤诱导方法，如手动浸入或热金属接触，存在高度变异性、可重复性有限且依赖操作者，降低了它们的转化相关性。本研究介绍了RAPID-3D（大鼠打印诱导装置-3D），这是一种新型的3D打印系统，旨在在大鼠模型中诱导均匀且可重复产生的烫伤，确保精确的暴露控制并将变异性降至最低。

方法

RAPID-3D具有四个烧伤暴露窗口（每个窗口为10×20毫米，间距为10毫米），在固定麻醉大鼠背部的同时，可控制暴露于沸水（100°C，8秒）。10只雌性Wistar大鼠每只接受8次受控烧伤。每只大鼠均设有内部未烧伤对照区域用于动物内比较。在烧伤后1小时使用数字平面测量法、组织学评估和通过激光多普勒线扫描（LDLS）进行实时微血管灌注分析来评估烧伤演变情况，并在烧伤后第4天、第9天和第21天重复进行。

结果

RAPID-3D在所有大鼠中产生了高度一致的烧伤面积（198±3.54毫米），动物间变异性低。组织学分析证实了全层表皮坏死和深部部分厚度真皮损伤（深度为600-900微米）。微血管趋势：烧伤后灌注立即下降，在第4天保持较低水平，并从第9天开始逐渐增加，表明新生血管形成和血管重塑在逐渐进行。RAPID-3D提供了一个标准化、可重复且与临床相关的烫伤模型，消除了操作者引起的变异性，增强了实验一致性，并为烧伤治疗开发和伤口愈合研究提供了强大的转化相关性。