Chen Liang, Wu Qiao, Yang Chen, Xin Xiulan, Xu Zhaochu, Luo Shuai, Liang Hao
School of Bioengineering, Beijing Polytechnic University, Beijing 100176, China.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Molecules. 2025 Jul 11;30(14):2940. doi: 10.3390/molecules30142940.
Natural products possess potent pharmacological activities and health benefits. However, drawbacks such as water insolubility, poor stability, and low bioavailability limit their practical applications. This research is dedicated to the development of suitable natural self-assembled nano-delivery systems to encapsulate natural molecule drugs, improving their dispersion and stability in aqueous solution. As a model drug, curcumin (Cur) was encapsulated in zinc-adenine nanoparticles (Zn-Adenine), based on the self-assembly of a coordination matrix material. Hyaluronic acid (HA) was further functionalized on the surface of Cur@(Zn-Adenine) to realize a tumor-targeted delivery system. The morphology was characterized through TEM and zeta potential analyses, while the encapsulation mechanism of the nanoparticles was researched via XRD and FTIR. The formed Cur@(Zn-Adenine)@HA nanoparticles exhibited good drug loading efficiency and drug loading rate. Moreover, compared to free Cur, Cur-loaded (Zn-Adenine)@HA showed enhanced pH stability and thermal stability. In particular, Cur@(Zn-Adenine)@HA demonstrated excellent biocompatibility and strong specificity for targeting CD44 protein on cancer cells. The above results indicate that (Zn-Adenine)@HA NPs can serve as an effective nano-delivery system for hydrophobic substances.
天然产物具有强大的药理活性和健康益处。然而，诸如水不溶性、稳定性差和生物利用度低等缺点限制了它们的实际应用。本研究致力于开发合适的天然自组装纳米递送系统，以包封天然分子药物，提高其在水溶液中的分散性和稳定性。作为模型药物，姜黄素（Cur）基于配位基质材料的自组装被包封在锌-腺嘌呤纳米颗粒（Zn-腺嘌呤）中。透明质酸（HA）进一步在Cur@(Zn-腺嘌呤)表面功能化，以实现肿瘤靶向递送系统。通过透射电子显微镜（TEM）和zeta电位分析对形态进行了表征，同时通过X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）研究了纳米颗粒的包封机制。形成的Cur@(Zn-腺嘌呤)@HA纳米颗粒表现出良好的载药效率和载药率。此外，与游离Cur相比，负载Cur的(Zn-腺嘌呤)@HA表现出增强的pH稳定性和热稳定性。特别地，Cur@(Zn-腺嘌呤)@HA对癌细胞上的CD44蛋白表现出优异的生物相容性和强特异性。上述结果表明，(Zn-腺嘌呤)@HA纳米颗粒可作为一种有效的疏水性物质纳米递送系统。
