Nechita Mihaela-Ancuța, Pârvu Alina Elena, Uifălean Ana, Iurian Sonia, Olah Neli-Kinga, Bab Timea Henrietta, Vârban Rodica, Nechita Vlad-Ionuț, Toiu Anca, Oniga Ovidiu, Benedec Daniela, Hanganu Daniela, Oniga Ilioara
Department of Pharmacognosy, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, Ion Creangă Street, 12, 400010 Cluj-Napoca, Romania.
Department of Morpho-Functional Sciences, Discipline of Pathophysiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.
Plants (Basel). 2025 Jul 9;14(14):2122. doi: 10.3390/plants14142122.
This study offers a detailed assessment of the polyphenolic composition and antioxidant, anti-inflammatory, and cardioprotective properties of lyophilized extracts derived from the aerial parts of and . The polyphenolic content was determined through the quantification of total polyphenols, flavonoids, and caffeic acid derivatives, complemented by LC-MS profiling. The antioxidant activity was evaluated in vitro using DPPH and FRAP assays, while the in vivo antioxidant and anti-inflammatory effects were investigated in a rat model of turpentine-oil-induced acute inflammation. Cardioprotective potential was assessed in a separate rat model of isoprenaline-induced myocardial infarction. Phytochemical analysis revealed a complex polyphenolic profile for both species, with tilianin and rosmarinic acid identified as predominant compounds. In the DPPH assay, both extracts exhibited marked radical scavenging activity (IC: 65.91 ± 1.21 μg/mL for ; 68.64 ± 2.48 μg/mL for ). In the in vivo assays, the administration of the extracts significantly decreased pro-oxidant biomarkers (TOS, OSI, MDA, NO) and enhanced antioxidant markers (TAC, SH groups). Furthermore, the extracts led to a significant reduction in serum levels of GOT, GPT, and CK-MB in rats subjected to myocardial injury, supporting their cardioprotective efficacy. Overall, the results suggest that and represent promising natural sources of polyphenolic compounds with potential therapeutic value in oxidative-stress-related inflammatory and cardiovascular disorders.
本研究对[两种植物名称]地上部分冻干提取物的多酚成分、抗氧化、抗炎和心脏保护特性进行了详细评估。通过对总多酚、黄酮类化合物和咖啡酸衍生物的定量分析，并辅以液相色谱 - 质谱联用（LC - MS）分析来确定多酚含量。体外使用DPPH和FRAP试验评估抗氧化活性，同时在松节油诱导的急性炎症大鼠模型中研究体内抗氧化和抗炎作用。在异丙肾上腺素诱导的心肌梗死大鼠模型中评估心脏保护潜力。植物化学分析表明两种植物均具有复杂的多酚谱，其中田基黄苷和迷迭香酸被确定为主要化合物。在DPPH试验中，两种提取物均表现出显著的自由基清除活性（[第一种植物提取物]的IC：65.91±1.21μg/mL；[第二种植物提取物]的IC：68.64±2.48μg/mL）。在体内试验中，提取物的给药显著降低了促氧化生物标志物（TOS、OSI、MDA、NO）并增强了抗氧化标志物（TAC、SH基团）。此外，提取物使心肌损伤大鼠的血清谷草转氨酶（GOT）、谷丙转氨酶（GPT）和肌酸激酶同工酶（CK - MB）水平显著降低，支持了它们的心脏保护功效。总体而言，结果表明[两种植物名称]是多酚化合物的有前景的天然来源，在与氧化应激相关的炎症和心血管疾病中具有潜在治疗价值。
