Țicolea Mădălina, Pop Raluca Maria, Pârvu Marcel, Usatiuc Lia-Oxana, Uifălean Ana, Pop Dalina Diana, Fischer-Fodor Eva, Ranga Floricuța, Rusu Crina Claudia, Cătoi Adriana Florinela, Palma-Garcia Francisco, Gherman Luciana-Mădălina, Pârvu Alina Elena
Department of Morpho-Functional Sciences, Discipline of Pathophysiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.
Department of Morpho-Functional Sciences, Discipline of Pharmacology, Toxicology and Clinical Pharmacology, "Iuliu Haţieganu" University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania.
Plants (Basel). 2025 Mar 26;14(7):1029. doi: 10.3390/plants14071029.
This study investigates the phytochemical composition, anti-inflammatory, antioxidant, and antiproliferative activities of and flowers and leaf ethanol extracts in acute rat inflammation model. Polyphenolic compounds were analyzed quantitatively (total phenolic (TPC) and total flavonoids (TFCs)) and qualitatively by HPLC-ESI MS analysis. The antioxidant activity was evaluated in vitro (by DPPH, FRAP, HO, and NO scavenging tests), and in vivo (by total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), and key oxidative damage markers). Inflammation was evaluated via nuclear factor-kB-p65 (NfkB-p65), and canonical NLRP3 inflammasome activation (with IL-1β, IL-18, caspase-1, and gasdermin D). The antiproliferative activity against human ovarian tumor cells (A2780cis, OVCAR-3, and OAW-42) was evaluated by the MTT assay, focusing on the modulation of multidrug resistance (MDR) pumps and the PARP-1 enzyme. Liver and renal toxicity were tested by measuring transaminases (ALT and AST), creatinine, and urea. The study results indicated that and flowers and leaf ethanol extracts have rich polyphenol content and moderate in vitro antioxidant activity. Tested extracts display an important antiproliferative activity against the ovarian tumor cell lines A2780cis, OVCAR-3, and OAW-42 based on chemoresistance countering and apoptotic mechanisms. There were differences related to the cell type and plant extract type. The tested plant extracts had significant and dose-dependent in vivo anti-inflammatory and antioxidant activity, with the flowers extract having the lowest efficiency. The anti-inflammatory and antioxidant activity biomarkers correlated with the extracts' chemical composition. There was no inflammation-induced hepatotoxicity, but renal dysfunction was associated. Only AANL improved the renal function. These results can be used to design and develop remedies with combined anti-inflammatory, antioxidant, and anti-proliferative activities.
本研究在急性大鼠炎症模型中，研究了[植物名称]花和叶乙醇提取物的植物化学成分、抗炎、抗氧化和抗增殖活性。通过高效液相色谱-电喷雾质谱分析对多酚类化合物进行了定量分析（总酚含量（TPC）和总黄酮含量（TFCs））和定性分析。体外评估抗氧化活性（通过DPPH、FRAP、HO和NO清除试验），体内评估（通过总氧化状态（TOS）、总抗氧化能力（TAC）、氧化应激指数（OSI）和关键氧化损伤标志物）。通过核因子-κB-p65（NfkB-p65）和经典NLRP3炎性小体激活（通过IL-1β、IL-18、半胱天冬酶-1和gasdermin D）评估炎症。通过MTT法评估对人卵巢肿瘤细胞（A2780cis、OVCAR-3和OAW-42）的抗增殖活性，重点关注多药耐药（MDR）泵和PARP-1酶的调节。通过测量转氨酶（ALT和AST）、肌酐和尿素来测试肝毒性和肾毒性。研究结果表明，[植物名称]花和叶乙醇提取物含有丰富的多酚含量和适度的体外抗氧化活性。基于化疗耐药性对抗和凋亡机制，测试提取物对卵巢肿瘤细胞系A2780cis、OVCAR-3和OAW-42显示出重要的抗增殖活性。细胞类型和植物提取物类型存在差异。测试的植物提取物具有显著的剂量依赖性体内抗炎和抗氧化活性，其中[植物名称]花提取物的效率最低。抗炎和抗氧化活性生物标志物与提取物的化学成分相关。没有炎症诱导的肝毒性，但与肾功能障碍有关。只有[植物名称]改善了肾功能。这些结果可用于设计和开发具有抗炎、抗氧化和抗增殖活性组合的药物。
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