Pastor Kristian, Nastić Nataša, Gavarić Aleksandra, Simić Siniša, Lončarić Ante, Banožić Marija, Aladić Krunoslav, Jokić Stela, Vladić Jelena
Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, Croatia.
Plants (Basel). 2025 Jul 17;14(14):2214. doi: 10.3390/plants14142214.
This study investigated the potential of high-voltage electrical discharge (HVED), as a green, non-thermal extraction technology, for recovering polyphenols from winter savory ( L.). Key process parameters, including frequency (40, 70, 100 Hz) and extraction time (1, 5, 15, 30, 45 min), were optimized, using water as a solvent and maintaining a constant solid-to-liquid ratio of 1:100 g/mL. The extracts were characterized for total polyphenol content (TPC), total flavonoid content (TFC), and antioxidant activity (DPPH, ABTS, FRAP), while individual phenolics were quantified via HPLC-DAD. Multivariate chemometric analyses, including Pearson correlation, heatmap clustering, and principal component analysis (PCA), were employed to reveal relationships between extraction conditions, polyphenolic profiles, and antioxidant activities. The results showed strong correlations between TPC, TFC, and antioxidant activity, with compounds such as quercetin-3-D-galactoside, procyanidin A2, and rutin identified as key contributors. Among the tested conditions, extraction at 70 Hz for 45 min provided the highest polyphenol yield and bioactivity. The application of HVED demonstrated its potential as an efficient and environmentally friendly technique for obtaining phenolic-rich extracts. In addition, the use of chemometric tools provided useful insights for optimizing extraction conditions and understanding the contributions of specific compounds to bioactivity. These results support future applications in clean-label product development and contribute to broader efforts in sustainable ingredient production for the food, cosmetic, and nutraceutical sectors.
本研究考察了高压放电（HVED）作为一种绿色非热提取技术从冬香薄荷（L.）中回收多酚的潜力。以水为溶剂，保持固液比为1:100 g/mL恒定，对包括频率（40、70、100 Hz）和提取时间（1、5、15、30、45分钟）在内的关键工艺参数进行了优化。对提取物的总多酚含量（TPC）、总黄酮含量（TFC）和抗氧化活性（DPPH、ABTS、FRAP）进行了表征，同时通过HPLC-DAD对单个酚类物质进行了定量。采用多元化学计量分析，包括皮尔逊相关性、热图聚类和主成分分析（PCA），以揭示提取条件、多酚谱和抗氧化活性之间的关系。结果表明，TPC、TFC和抗氧化活性之间存在强相关性，槲皮素-3-D-半乳糖苷、原花青素A2和芦丁等化合物被确定为关键贡献者。在测试条件中，70 Hz提取45分钟可获得最高的多酚产量和生物活性。HVED的应用证明了其作为一种高效且环保的技术来获得富含酚类提取物的潜力。此外，化学计量工具的使用为优化提取条件和理解特定化合物对生物活性的贡献提供了有用的见解。这些结果支持了其在清洁标签产品开发中的未来应用，并为食品、化妆品和营养保健品行业可持续成分生产的更广泛努力做出了贡献。
