Zheng Yimei, Chen Tonglin, Li Ke, Wang Liang, Liu Xuwei, Zhao Lei, Hu Zhuoyan, Wang Kai
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
Carbohydr Polym. 2025 Oct 15;366:123904. doi: 10.1016/j.carbpol.2025.123904. Epub 2025 Jun 15.
Galloyl-based polyphenols including gallic acid (GA) and tannic acid (TA) were used to form complexes with high-amylose maize starch (HAMS). The relationship among complex structure, starch digestibility in vitro, and postprandial glycemic response, hormone concentration and satiety in mice were investigated. Both GA and TA interacted with starch through noncovalent bonds and formed complexes with V-type crystalline structure. The polyphenol loading content in HAMS-TA was higher than HAMS-GA, probably because TA has more galloyl moieties. In vitro digestion revealed HAMS-TA/GA complexes had lower digestion rate (k) and proportion of digestible starch (C) than control samples, attributing to their higher crystallinity and inhibition on α-amylase. HAMS-TA had lower k and C than HAMS-GA, resulted from its higher polyphenol loading content. In vivo digestion in mice indicated complexes had lower glycemic response, higher concentrations of GLP-1 and PYY than control groups. They also showed good ability in decreasing glycemic elevation after a subsequent meal and enhancing satiety. HAMS-TA exhibited better performance than HAMS-GA. The in vitro and in vivo results are in good agreements, suggesting the potential ability of starch-galloyl-based polyphenol complexes, especially HAMS-TA complex, in preventing type-2 diabetes and obesity. These would provide guidance for developing starchy foods with functional properties.
基于没食子酰基的多酚，包括没食子酸（GA）和单宁酸（TA），被用于与高直链玉米淀粉（HAMS）形成复合物。研究了复合物结构、体外淀粉消化率以及小鼠餐后血糖反应、激素浓度和饱腹感之间的关系。GA和TA均通过非共价键与淀粉相互作用，并形成具有V型晶体结构的复合物。HAMS-TA中的多酚负载量高于HAMS-GA，这可能是因为TA具有更多的没食子酰基部分。体外消化显示，HAMS-TA/GA复合物的消化率（k）和可消化淀粉比例（C）低于对照样品，这归因于它们更高的结晶度和对α-淀粉酶的抑制作用。HAMS-TA的k和C低于HAMS-GA，这是由于其更高的多酚负载量。小鼠体内消化表明，复合物的血糖反应低于对照组，胰高血糖素样肽-1（GLP-1）和酪酪肽（PYY）浓度高于对照组。它们还显示出在降低后续餐食后的血糖升高和增强饱腹感方面具有良好的能力。HAMS-TA的表现优于HAMS-GA。体外和体内结果吻合良好，表明基于淀粉-没食子酰基多酚的复合物，尤其是HAMS-TA复合物，在预防2型糖尿病和肥胖方面具有潜在能力。这些将为开发具有功能特性的淀粉类食品提供指导。
