Kheradmand Mohammadreza, Saberpour Masoumeh, Bakhshi Bita, Bahroudi Mahboube
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Influenza and Other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran.
Can J Infect Dis Med Microbiol. 2025 Jul 22;2025:9936375. doi: 10.1155/cjid/9936375. eCollection 2025.
Cholera remains a global challenge, and understanding how . adapts to environmental condition is essential for innovating new management strategies. This research aims to examine the expression of , , and genes in . (El Tor and classical biotypes) after interaction with Caco-2 cells compared to brain heart infusion (BHI) broth. After assaying of viability of Caco-2 cells against . at multiplicity of infection (MOI) of 10, 20, 50, and 80, the number of bacteria attached to Caco-2 cells was determined using the adhesion assay. To conduct a valid comparison, an equivalent number of bacteria that attached to Caco-2 cells was inoculated into BHI broth. The expression of , , and genes in . (El Tor and classical biotypes) was assayed using the real-time PCR method. After interaction of Caco-2 cells with . , the expression of the genes , , and in the El Tor biotype increased by 2-, 1.02-, and 7-fold, respectively, while in the classical biotype, it increased by 6-, 2-, and 13-fold, respectively. The expression of the genes and was significantly increased in Caco-2 cells in both biotypes. There was a significant increase in the expression of the gene in the classical biotype, while no significant increase was detected in the expression of this gene in the El Tor biotype. Caco-2 cells induced the highest increase in gene expression in the classical biotype, while there was no significant effect on gene expression in the El Tor biotype of . . In conclusion, . showed greater pathogenicity in the Caco-2 cells. Knowing the behavior of . in different conditions can lead to create innovative strategies for combating and managing cholera.
霍乱仍然是一项全球性挑战，了解[细菌名称]如何适应环境条件对于创新新的管理策略至关重要。本研究旨在检测[细菌名称]（埃尔托生物型和古典生物型）与Caco-2细胞相互作用后，与脑心浸液（BHI）肉汤相比，[相关基因名称]、[相关基因名称]和[相关基因名称]基因的表达情况。在检测Caco-2细胞在感染复数（MOI）为10、20、50和80时对[细菌名称]的活力后，使用黏附试验确定附着在Caco-2细胞上的细菌数量。为了进行有效比较，将等量附着在Caco-2细胞上的细菌接种到BHI肉汤中。使用实时PCR方法检测[细菌名称]（埃尔托生物型和古典生物型）中[相关基因名称]、[相关基因名称]和[相关基因名称]基因的表达。Caco-2细胞与[细菌名称]相互作用后，埃尔托生物型中[相关基因名称]、[相关基因名称]和[相关基因名称]基因的表达分别增加了2倍、1.02倍和7倍，而在古典生物型中，分别增加了6倍、2倍和13倍。两种生物型的Caco-2细胞中[相关基因名称]和[相关基因名称]基因的表达均显著增加。古典生物型中[相关基因名称]基因的表达显著增加，而在埃尔托生物型中未检测到该基因表达的显著增加。Caco-2细胞在古典生物型中诱导[相关基因名称]基因表达增加最多，而对[细菌名称]埃尔托生物型中的[相关基因名称]基因表达没有显著影响。总之，[细菌名称]在Caco-2细胞中表现出更大的致病性。了解[细菌名称]在不同条件下的行为可以为对抗和管理霍乱创造创新策略。
