Tripathy B C, Rebeiz C A
Anal Biochem. 1985 Aug 15;149(1):43-61. doi: 10.1016/0003-2697(85)90475-0.
General equations which permit the determination of the amounts of any two closely related fluorescent compounds which can be distinguished by 77 degrees K but not by 293 degrees K spectrofluorometry have been described. This was achieved in the presence or absence of a third interfering compound, without prior separation of the fluorescent species. The adaptation of the generalized equations to the determination of the amounts of monovinyl (MV) and divinyl (DV) Mg-protoporphyrins or of MV and DV protochlorophyll(ides) in the presence or absence of Mg-Protos [Mg-protoporphyrin IX (Mg-Proto), Mg-Proto monoester, Mg-Proto diester or a mixture of those three tetrapyrroles] interference, was then demonstrated over a wide range of MV/DV tetrapyrrole proportions. These equations are likely to be very useful for the study of the intermediary metabolism of the monovinyl and divinyl chlorophyll biosynthetic routes in plants.
已经描述了一些通用方程，这些方程可以确定任何两种密切相关的荧光化合物的含量，这两种化合物可以通过77K的温度区分，但不能通过293K的荧光光谱法区分。这是在有或没有第三种干扰化合物的情况下实现的，无需事先分离荧光物质。然后证明了在有或没有Mg-Protos[镁原卟啉IX（Mg-Proto）、Mg-Proto单酯、Mg-Proto二酯或这三种四吡咯的混合物]干扰的情况下，通用方程适用于测定单乙烯基（MV）和二乙烯基（DV）镁原卟啉或MV和DV原叶绿素（酯）的含量。在广泛的MV/DV四吡咯比例范围内，这些方程可能对研究植物中单乙烯基和二乙烯基叶绿素生物合成途径的中间代谢非常有用。
