Belanger F C, Rebeiz C A
J Biol Chem. 1982 Feb 10;257(3):1360-71.
It is shown that the magnesium-protoporphyrin-6-ester pool of plants is made up of monovinyl and divinyl components which were separated by chromatography on thin layers of polyethylene. At 77K, in ether, monovinyl-Mg-protoporphyrin-6-ester exhibited fluorescence emission and excitation maxima at 589 and 417 nm, respectively. The divinyl-Mg-protoporphyrin-6-ester component exhibited red-shifted emission and excitation maxima at 591 and 424 nm respectively. Demetallation of the monovinyl and divinyl Mg-protoporphyrin ester components converted them into monovinyl and divinyl-protoporphyrin IX free bases. Catalytic Mg-mesoporphyrin ester and the demetallated free bases into mesoporphyrin ester, Monovinyl-Mg-porphyrin chemical species were also detected in the Mg-protoporphyrin IX and the Mg-protoporphyrin IX diester pools of plants. It is proposed that the Mg-protoporphyrin IX, Mg-protoporphyrin IX-6-ester, and Mg-protoporphyrin IX-diester pools of plants are heterogenous and are made up of monovinyl and divinyl chemical species.
结果表明，植物的镁原卟啉-6-酯库由单乙烯基和二乙烯基成分组成，它们通过在聚乙烯薄层上的色谱法分离。在77K的乙醚中，单乙烯基-Mg-原卟啉-6-酯的荧光发射和激发最大值分别在589和417nm处。二乙烯基-Mg-原卟啉-6-酯成分的发射和激发最大值分别在591和424nm处出现红移。单乙烯基和二乙烯基Mg-原卟啉酯成分的脱金属作用将它们转化为单乙烯基和二乙烯基-原卟啉IX游离碱。催化Mg-中卟啉酯和脱金属的游离碱转化为中卟啉酯，在植物的Mg-原卟啉IX和Mg-原卟啉IX二酯库中也检测到单乙烯基-Mg-卟啉化学物质。有人提出，植物的Mg-原卟啉IX、Mg-原卟啉IX-6-酯和Mg-原卟啉IX-二酯库是异质的，由单乙烯基和二乙烯基化学物质组成。
