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Quantification of sulfated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue.

作者信息

Enobakhare B O, Bader D L, Lee D A

机构信息

IRC in Biomedical Materials, UCL, Stanmore, Middlesex, United Kingdom.

出版信息

Anal Biochem. 1996 Dec 1;243(1):189-91. doi: 10.1006/abio.1996.0502.

DOI:10.1006/abio.1996.0502
PMID:8954546
Abstract
摘要

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1
Quantification of sulfated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue.
Anal Biochem. 1996 Dec 1;243(1):189-91. doi: 10.1006/abio.1996.0502.
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Automation of 1,9-dimethylmethylene blue dye-binding assay for sulfated glycosaminoglycans with application to cartilage microcultures.用于硫酸化糖胺聚糖的1,9 - 二甲基亚甲基蓝染料结合测定法的自动化及其在软骨微培养中的应用。
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A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.软骨培养物中硫酸化糖胺聚糖的直接分光光度微量测定法。
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[Indirect spectrophotometric microdetermination of sulfated glycosaminoglycans with dimethylmethylene blue].[用二甲基亚甲基蓝间接分光光度法微量测定硫酸化糖胺聚糖]
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Fact versus artifact: avoiding erroneous estimates of sulfated glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue-engineered constructs.事实与假象：使用二甲基亚甲基蓝比色法检测组织工程构建体时避免硫酸化糖胺聚糖含量的错误估计
Eur Cell Mater. 2015 Apr 19;29:224-36; discussion 236. doi: 10.22203/ecm.v029a17.
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The basis and applicability of the dimethylmethylene blue binding assay for sulfated glycosaminoglycans.用于硫酸化糖胺聚糖的二甲基亚甲基蓝结合测定法的基础及适用性。
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Use of polyvinyl alcohol to stabilize binding of sulfated glycosaminoglycans to dimethylmethylene blue.
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Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue.使用二甲基亚甲基蓝改进硫酸化糖胺聚糖的定量和鉴别
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