Rikihisa Y, Lin Y C, Walton A
Anat Rec. 1985 Sep;213(1):72-6. doi: 10.1002/ar.1092130110.
Intramuscular injections of gossypol acetic acid (25 mg in 10% EtOH/kg/day beginning on day 2 of diestrus) disrupted early pregnancy in rats as determined by light and electron microscopy. As in pregnant controls, in the uteri of treated rats increased glandular secretion, stromal hyperemia, and decidual tissue formation were noted at days 3-5 of pregnancy. At day 6, extreme hyperemia and stromal hemorrhage had occurred around well-developed decidual tissue with foci of denuded mucosal surface. There was extravasation of blood into the uterine lumen, which was absent in controls. At days 5 and 6 of pregnancy, electron microscopy revealed shorter and fewer microvilli on the uterine glandular cells in the treated versus the control uterus. Luminal epithelial cells had not undergone the normal changes of pregnancy. These results imply that gossypol administered under our conditions neither prevented nor delayed implantation and formation of decidual tissue in the rat uterine endometrium but continuing development of the endometrium was disrupted at day 6 of pregnancy. This disruption of pregnancy may have resulted from a luteolytic action by gossypol that would not permit full structural differentiation in the rat uterus after implantation.
通过光学显微镜和电子显微镜观察发现，在动情后期第2天开始，每天给大鼠肌肉注射醋酸棉酚（25毫克/10%乙醇/千克体重）会导致大鼠早期妊娠中断。与正常妊娠对照组一样，在妊娠第3 - 5天，处理组大鼠子宫内可见腺体分泌增加、间质充血以及蜕膜组织形成。在妊娠第6天，发育良好的蜕膜组织周围出现极度充血和间质出血，伴有黏膜表面剥脱灶。血液渗入子宫腔，而对照组未见此现象。在妊娠第5天和第6天，电子显微镜观察显示，与对照子宫相比，处理组大鼠子宫腺上皮细胞的微绒毛更短且数量更少。腔上皮细胞未发生正常的妊娠变化。这些结果表明，在我们的实验条件下给予棉酚，既不能阻止也不能延迟大鼠子宫内膜的着床和蜕膜组织形成，但在妊娠第6天，子宫内膜的持续发育受到破坏。这种妊娠中断可能是由于棉酚的溶黄体作用，使得大鼠子宫在着床后无法进行充分的结构分化。
