Hong S L, Robbers J E
Appl Environ Microbiol. 1985 Sep;50(3):558-61. doi: 10.1128/aem.50.3.558-561.1985.
Auxotrophic, spore color, and alkaloid biosynthetic mutants of Penicillium roquefortii were selected after N-methyl-N'-nitro-N-nitrosoguanidine treatment. Diploids were obtained via protoplast fusion techniques, and the segregants from a diploid were genetically analyzed. The data demonstrated the potential of parasexual recombination in this organism. Evidence was obtained which suggests that the his and sts (sensitivity to Sulfatase) genes may be linked. The genetic information obtained in this study can serve as a starting point for further mapping of genes in P. roquefortii, and indications are that this organism may serve as a promising vehicle for the genetic study of the formation of ergoline alkaloids.
在用N-甲基-N'-硝基-N-亚硝基胍处理后，挑选出了罗克福特青霉的营养缺陷型、孢子颜色和生物碱生物合成突变体。通过原生质体融合技术获得了二倍体，并对一个二倍体的分离后代进行了遗传分析。数据证明了该生物体中准性重组的潜力。获得的证据表明，组氨酸和sts（对硫酸酯酶敏感）基因可能是连锁的。本研究中获得的遗传信息可作为进一步绘制罗克福特青霉基因图谱的起点，并且有迹象表明该生物体可能成为麦角灵生物碱形成遗传研究的一个有前景的载体。
