Durand N, Reymond P, Fèvre M
Laboratoire de Biologie Cellulaire Fongique, UMR CNRS 106, Université Lyon I (Bât. 405), Villeurbanne, France.
Curr Genet. 1993 Nov;24(5):417-20. doi: 10.1007/BF00351850.
Random amplified polymorphic DNAs (RAPDs) were used as a genetic marker system to characterize recombinant strains following the parasexual cycle of Penicillium roqueforti. After protoplast fusion and haploidization of diploid hybrids, segregants characterized by a reassortment of the parental genetic markers displayed specific RAPD fingerprints. The appearance or the loss of RAPD fragments demonstrate that these markers provide an efficient method to analyze recombination and to characterize somatic hybrids.
随机扩增多态性DNA（RAPD）被用作一种遗传标记系统，以表征罗克福青霉准性循环后的重组菌株。在原生质体融合和二倍体杂种单倍体化后，以亲本遗传标记重新组合为特征的分离株显示出特定的RAPD指纹图谱。RAPD片段的出现或缺失表明，这些标记为分析重组和表征体细胞杂种提供了一种有效的方法。
