Gravino Matteo, Mugford Sam T, Pontiggia Daniela, Joyce Joshua, Drurey Claire, Prince David C, Cervone Felice, De Lorenzo Giulia, Hogenhout Saskia A
Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, Piazzale Aldo Moro, 5, 00185, Rome, Italy.
New Phytol. 2025 Oct;248(2):913-935. doi: 10.1111/nph.70419. Epub 2025 Jul 30.
Damage-associated molecular pattern (DAMP)-triggered immunity (DTI) serves as a crucial first line of defence against aphid attack, yet how aphids manage this response remains unclear. By investigating the colonisation of Arabidopsis thaliana by the highly polyphagous peach-potato aphid (Myzus persicae), we identified cell wall-derived DAMPs, specifically oligogalacturonides (OGs), as key in inducing DTI against aphids. The OG-responsive immune components BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1/BAK1-LIKE 1, CPK5/CPK6, GLYCINE-RICH PROTEIN 3, and EDS1 collectively contribute to DTI limiting aphid colonisation. We found that aphid feeding limits OG production in response to wounding. Additionally, the salivary chemosensory protein (CSP) effector Mp10/CSP4, which is known to be delivered into the cytoplasm of plant cells early in aphid attack, inhibits OG-induced DTI. While Mp10 suppresses OG-induced DTI, it also interacts with EDS1-mediated defences, enhancing aphid fecundity in the absence of EDS1 while restoring OG responsiveness, revealing its broader role in immune modulation and effector-driven host adaptation.
损伤相关分子模式（DAMP）触发的免疫（DTI）是抵御蚜虫攻击的关键第一道防线，但蚜虫如何应对这种反应仍不清楚。通过研究多食性桃蚜（Myzus persicae）对拟南芥的定殖，我们确定细胞壁衍生的DAMPs，特别是寡聚半乳糖醛酸（OGs），是诱导针对蚜虫的DTI的关键因素。对OG有反应的免疫成分油菜素类固醇不敏感1相关受体激酶1/类BAK1、CPK5/CPK6、富含甘氨酸的蛋白3和EDS1共同促成了限制蚜虫定殖的DTI。我们发现蚜虫取食会限制受伤后OG的产生。此外，唾液化学感应蛋白（CSP）效应子Mp10/CSP4，已知在蚜虫攻击早期被输送到植物细胞的细胞质中，会抑制OG诱导的DTI。虽然Mp10抑制OG诱导的DTI，但它也与EDS1介导的防御相互作用，在没有EDS1的情况下提高蚜虫的繁殖力，同时恢复OG反应性，揭示了其在免疫调节和效应子驱动的宿主适应中的更广泛作用。
