Zhang Yingdi, Sun Huijuan, Huang Qi, Zhang Lei, Zou Xin, Liu Yang
Department of Civil and Environmental Engineering, Faculty of Engineering, University of Alberta, Edmonton, Alberta, Canada.
School of Civil and Environmental Engineering, Queensland University of Technology, Brisbane, Queensland, Australia.
Water Environ Res. 2025 Aug;97(8):e70153. doi: 10.1002/wer.70153.
Anaerobic digestion (AD) is a promising technology for treating high-strength industrial wastewater while recovering biogas as a renewable energy source. In this study, a pilot-scale up-flow anaerobic sludge blanket (UASB) reactor, with a working volume of 225 L, was operated for 130 days to treat raw distillery wastewater. Three different types of distillery wastewater, including centrate, spent caustic, and other low-strength process wastewater generated during operations (e.g., condensation water), were mixed to balance extreme pH levels and create conditions more suitable for AD. The UASB reactor demonstrated stable performance at an organic loading rate (OLR) of 10.0 kg COD/m/day, achieving a COD removal efficiency of 86%. Average methane yield was more than 71% throughout the operation. Microbial community analysis revealed a significant increase in key syntrophic bacteria and methanogens, with Methanobacterium accounting for 55.8% of the archaeal population. Network and Mantel analyses indicated that syntrophic partnerships play a crucial role in enhancing AD of distillery wastewater under high OLR conditions. Overall, the UASB reactor exhibited high process stability, highlighting its potential for large-scale application in distillery wastewater treatment.
厌氧消化（AD）是一种很有前景的技术，可用于处理高强度工业废水，同时回收沼气作为可再生能源。在本研究中，一个工作体积为225升的中试规模上流式厌氧污泥床（UASB）反应器运行了130天，用于处理原酒厂废水。将三种不同类型的酒厂废水，包括离心液、废碱液和运行过程中产生的其他低强度工艺废水（如冷凝水）混合，以平衡极端的pH值，并创造更适合厌氧消化的条件。UASB反应器在有机负荷率（OLR）为10.0 kg COD/m³/天的情况下表现出稳定的性能，COD去除效率达到86%。在整个运行过程中，平均甲烷产率超过71%。微生物群落分析显示，关键的互营细菌和产甲烷菌显著增加，其中甲烷杆菌占古菌种群的55.8%。网络分析和Mantel分析表明，在高OLR条件下，互营伙伴关系在提高酒厂废水厌氧消化效率方面起着关键作用。总体而言，UASB反应器表现出很高的工艺稳定性，突出了其在酒厂废水处理大规模应用中的潜力。
