Thungmungmee Suradwadee, Wisidsri Nakuntwalai
Faculty of Integrative Medicine, Rajamangala University of Technology Thanyaburi, Thanyaburi, Pathum Thani, 12130, Thailand.
Sci Rep. 2025 Jul 31;15(1):28006. doi: 10.1038/s41598-025-13679-9.
This study investigates the anti-photoaging potential of ethanolic extracts from avocado peel and seed and their application in a nanoemulgel formulation. Both extracts demonstrated high phenolic content and strong antioxidant activity, with DPPH IC values of 57.05 ± 1.65 µg/ml (peel) and 52.44 ± 0.32 µg/ml (seed). In UVB-irradiated human dermal fibroblasts (BJ cells), the extracts (12.5-50 µg/ml) significantly reduced intracellular ROS, decreased MMP-1 and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and restored collagen levels. A nanoemulgel was developed using the phase inversion composition (PIC) method, with avocado oil, isononyl isononanoate, Tween 60, and polyglyceryl-3 diisostearate, which resulted in nanoemulsions (NEs) with an average droplet size of 84.45 ± 5.45 nm and a zeta potential of -69.94 ± 5.66 mV. Incorporation of 0.5% Aqupec HV-505E and avocado extracts produced stable, light green gel. The resulting avocado extract-loaded nanoemulgel (ANE) demonstrated antioxidant activity, cytocompatibility with BJ cells, and stability in terms of color, pH, and viscosity. These findings reveal the anti-aging potential of avocado peel and seed extracts in preserving effectiveness within nanoemulgel for skin applications.
本研究调查了鳄梨果皮和种子乙醇提取物的抗光老化潜力及其在纳米乳凝胶制剂中的应用。两种提取物均显示出高酚含量和强抗氧化活性，DPPH IC50值分别为57.05±1.65μg/ml（果皮）和52.44±0.32μg/ml（种子）。在紫外线B照射的人皮肤成纤维细胞（BJ细胞）中，提取物（12.5 - 50μg/ml）显著降低细胞内活性氧，减少基质金属蛋白酶-1和促炎细胞因子（肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6），并恢复胶原蛋白水平。使用相转变组成（PIC）法制备了一种纳米乳凝胶，其成分包括鳄梨油、异壬酸异壬酯、吐温60和聚甘油-3二异硬脂酸酯，得到平均液滴尺寸为84.45±5.45nm、zeta电位为-69.94±5.66mV的纳米乳液（NEs）。加入0.5%的Aqupec HV - 505E和鳄梨提取物可制成稳定的浅绿色凝胶。所得负载鳄梨提取物的纳米乳凝胶（ANE）具有抗氧化活性、与BJ细胞的细胞相容性以及在颜色、pH值和粘度方面的稳定性。这些发现揭示了鳄梨果皮和种子提取物在纳米乳凝胶中用于皮肤应用时保持有效性的抗老化潜力。
