Enany Mohamed E, El-Mahallawy Heba S, Mohammed Wafaa E I, Hassan Wafaa M M, Aboueisha Abdelkarim M
Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Department of Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
BMC Microbiol. 2025 Jul 31;25(1):469. doi: 10.1186/s12866-025-04187-x.
() is an opportunistic pathogen belonging to family. It is present in the intestinal tract of animals and humans, and is considered as a major threat to public health. A total of 33 previously identified strains isolated from of broiler chicken ( = 20) and, humans ( = 13) were tested for their antimicrobial sensitivity and virulence genes detection.
The antimicrobial sensitivity testing of broiler chickens strains, showed complete phenotypic resistance towards ampicillin (100%), colistin (100%), fosfomycin (100%), trimethoprim/sulphamethaxole (100%) followed by gentamicin (90%), cefotaxime (90%) and chloramphenicol (75%). However, all the isolates were sensitive to imipenem (100%). human strains showed complete phenotypic resistance to fosfomycin (100%), followed by ampicillin (92.3%), colistin (92.3%), and ciprofloxacin and trimethoprim/sulfamethaxole (76.9%, each). The resistance gene profiling of the chicken strains was A (65%), A1 (65%), 1 (60%), A (50%), A (40%), and 1 (35%), and the most prevalent virulence gene was A (85%), that was detected either alone in 9 isolates (52.9%), or in combination with the A gene in 8 isolates (47.1%), and with H gene in 8 isolates (47.1%), followed by H (55%) and A genes (40%). On the other hand, the human strains gene profiling showed 1 (61.5%), A (53.8%), and 1 (46.2%) genes, while A1 was not detected. The most prevalent virulence genes in the human strains were A and A genes (46.2%, each), followed by H gene (38.5%). Overall, multidrug-resistant (MDR) (33.3%), extensively-drug resistant (XDR) (57.6%) and pan-drug resistant (PDR) (9.1%) isolates were detected.
The distribution of such virulent and resistant isolates among apparently healthy broiler chicken, highlights their potential role in the transmission of both resistance and virulence attributes to the surrounding environment and human contacts. Moreover, spreading of carbabenem resistant among human isolates, suggest the urgent need for more strict measures for the uncontrolled usage of antibiotics.
（）是属于某科的一种机会致病菌。它存在于动物和人类的肠道中，被认为是对公共卫生的重大威胁。对总共33株先前从肉鸡（n = 20）和人类（n = 13）中分离出的已鉴定菌株进行了抗菌敏感性测试和毒力基因检测。
肉鸡菌株的抗菌敏感性测试显示，对氨苄西林（100%）、黏菌素（100%）、磷霉素（100%）、甲氧苄啶/磺胺甲恶唑（100%）表现出完全的表型耐药，其次是庆大霉素（90%）、头孢噻肟（90%）和氯霉素（75%）。然而，所有分离株对亚胺培南（100%）敏感。人类菌株对磷霉素（100%）表现出完全的表型耐药，其次是氨苄西林（92.3%）、黏菌素（92.3%）以及环丙沙星和甲氧苄啶/磺胺甲恶唑（均为76.9%）。鸡菌株的耐药基因谱为A（65%）、A1（65%）、1（60%）、A（50%）、A（40%）和1（35%），最普遍的毒力基因是A（85%），其在9株分离株（52.9%）中单独检测到，或在8株分离株（47.1%）中与A基因联合检测到，在8株分离株（47.1%）中与H基因联合检测到，其次是H（55%）和A基因（40%）。另一方面，人类菌株的基因谱显示为1（61.5%）、A（53.8%）和1（46.2%）基因，而未检测到A1。人类菌株中最普遍的毒力基因是A和A基因（均为46.2%），其次是H基因（38.5%）。总体而言，检测到多重耐药（MDR）（33.3%）、广泛耐药（XDR）（57.6%）和泛耐药（PDR）（9.1%）分离株。
这种有毒力且耐药的分离株在看似健康的肉鸡中的分布，凸显了它们在将耐药性和毒力特性传播到周围环境及人类接触者方面的潜在作用。此外，碳青霉烯耐药菌在人类分离株中的传播，表明迫切需要采取更严格的措施来控制抗生素的无节制使用。
