Levin Daniel S, Cué Royo Camila S, Johnson Denis, Ghosh Soumalya, Balmuri Sricharani Rao, Usman Huda, Martínez Vásquez Shakira M, Yesudoss David Kumar, Djire Abdoulaye, Bedewy Mostafa, Niepa Tagbo H R
Pittsburgh Allderdice High School, Pittsburgh, PA, USA.
Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Biofilm. 2025 Jul 19;10:100305. doi: 10.1016/j.bioflm.2025.100305. eCollection 2025 Dec.
is the leading cause of skin infections in the U.S., and its rapid evolution and resistance to antibiotics create a barrier to effective treatment. In this study, we engineered a composite membrane with bacterial cellulose and carbon nanotubes (BC-CNT) as an electroactive dressing to rapidly eradicate vancomycin-intermediate . Nonpathogenic produced the BC membrane at an air-liquid interface. Then, carboxyl-functionalized multi-walled CNTs were integrated into decellularized BC to create stable and electrically conductive BC-CNT dressings. The electric potential and ionic flux across BC-CNT were modeled and standardized via chronoamperometry for experimental validation. We found that treatment with electroactive BC-CNT increases sensitivity to vancomycin and prevents macro-scale biofilm formation. The bactericidal efficacy of the composite membrane is consistent with electrochemical stress caused by voltage mediated with BC-CNT. After a single hour of combinatorial electrical and drug treatment, biofilm-forming capacity was inhibited by nearly 92 %. These results advance applications of electrochemistry in medicine and create a new direction to overcome infections on skin and soft tissues.
在美国，它是皮肤感染的主要原因，其快速进化和对抗生素的耐药性给有效治疗造成了障碍。在本研究中，我们设计了一种以细菌纤维素和碳纳米管（BC-CNT）为原料的复合膜作为电活性敷料，以快速根除万古霉素中介菌。非致病性细菌在气液界面产生BC膜。然后，将羧基功能化的多壁碳纳米管整合到脱细胞的BC中，以制备稳定且导电的BC-CNT敷料。通过计时电流法对BC-CNT上的电势和离子通量进行建模和标准化，以进行实验验证。我们发现，电活性BC-CNT处理可提高对万古霉素的敏感性，并防止大规模生物膜形成。复合膜的杀菌效果与BC-CNT介导的电压引起的电化学应力一致。经过一小时的电和药物联合治疗后，生物膜形成能力被抑制了近92%。这些结果推动了电化学在医学中的应用，并为克服皮肤和软组织感染开辟了新方向。
