Yu Rundong, Zhang Junwei, Peng Xiaohong, Chen Zhixing
State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, National Biomedical Imaging Center, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, College of Future Technology, Peking University, Beijing 100871, China.
Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Chem Biomed Imaging. 2025 Mar 4;3(7):455-461. doi: 10.1021/cbmi.4c00116. eCollection 2025 Jul 28.
Zinc biology significantly impacts human physiological and pathological processes, especially for β-cell endocrinology. In 2021, our group reported the family with minimal phototoxicity and μM affinities for monitoring Zn/insulin corelease during vesicular secretory events on β-cells (Zhang et al. Angew. Chem. Int. Ed.2021, 60 (49), 25846-25855). Here, we synthesized a series of Zn probes () featuring thiomorpholine monoxide auxochromes with values ranging from 160 nM to 94 μM. By inhibiting the twisted intramolecular charge transfer (TICT) state, the fluorescence quantum yields of can be effectively increased to ∼7 times those of . A privileged candidate, , has a high turn-on ratio (∼134), appropriate affinity (340 nM), and excellent hydrophilicity, making it a powerful tool for long-term recording of insulin secretion in mouse islets with minimal phototoxicity.
锌生物学对人类生理和病理过程有显著影响，尤其是对β细胞内分泌学。2021年，我们团队报道了一类具有最小光毒性和微摩尔亲和力的家族，用于监测β细胞囊泡分泌事件期间锌/胰岛素的共释放（Zhang等人，《德国应用化学》，2021年，60(49)，25846 - 25855）。在此，我们合成了一系列具有硫代吗啉酮助色团的锌探针（），其解离常数在160 nM至94 μM范围内。通过抑制扭曲的分子内电荷转移（TICT）状态，的荧光量子产率可有效提高至的约7倍。一个有优势的候选物，具有高开启比率（约134）、合适的亲和力（340 nM）和出色的亲水性，使其成为在小鼠胰岛中以最小光毒性长期记录胰岛素分泌的有力工具。
