使用冷冻组织分析发育中大鼠大脑中线粒体复合物I的活性和ATP合酶的ATP水解能力。
Analysis of mitochondrial Complex I activity and ATP hydrolysis capacity of ATP synthase in developing rat brains using frozen tissues.
作者信息
Yao Pamela J, Munk Rachel, Gorospe Myriam, Kapogiannis Dimitrios
机构信息
Laboratory of Clinical Investigation, National Institute on Aging Intramural Research Program, National Institutes of Health.
Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health.
出版信息
MicroPubl Biol. 2025 Jul 16;2025. doi: 10.17912/micropub.biology.001641. eCollection 2025.
In a previous study, we analyzed the activity of the mitochondrial respiratory Complex II, Complex IV and ATP synthase in frozen tissues of postnatal rat brains (Yao et al., 2023). In this study, we expand our capability of assessing mitochondrial functions using frozen tissue samples. We optimize protocols for measuring the activity of Complex I, and ATP hydrolysis capacity - known as the reverse action - of ATP synthase. We show that the specific functions of these mitochondrial proteins increase linearly as the brain develops.
在之前的一项研究中，我们分析了新生大鼠脑冷冻组织中线粒体呼吸复合物II、复合物IV和ATP合酶的活性（Yao等人，2023年）。在本研究中，我们扩展了使用冷冻组织样本评估线粒体功能的能力。我们优化了测量复合物I活性以及ATP合酶ATP水解能力（即所谓的反向作用）的实验方案。我们发现，随着大脑发育，这些线粒体蛋白的特定功能呈线性增加。
Zotero 插件