Gai Dandan, Li Xu, Lin Huaxiang, Yuan Rusheng, Long Jinlin, Lin Qun
College of Chemistry of Fuzhou University, State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, Fuzhou 350116, China.
Department of Anesthesia, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350002, China.
ACS Appl Bio Mater. 2025 Aug 18;8(8):7126-7138. doi: 10.1021/acsabm.5c00885. Epub 2025 Aug 1.
The CeO/CuMoO type II heterojunction with varying CeO contents was synthesized by the hydrothermal method. These heterojunction catalysts exhibit superior antibacterial activity against both and under visible light irradiation compared to CuMoO alone. The evaluations of bacterial morphology, intracellular content concentration of bacteria, and reactive oxygen species (ROS) during the photocatalytic process indicated that the ROS oxidize phospholipids in the bacterial cell membrane, which leads to the damage of the cell structure and leakage of cell contents, ultimately resulting in bacterial inactivation. Both EPR (Electron Paramagnetic Resonance) and ROS scavenging experiments confirmed that superoxide radicals (·O) and e play an important role in the photocatalytic antibacterial activity. The introduction of CeO into CuMoO enhances the heterojunction's photoelectric performance and promotes the efficient separation of photogenerated carriers, which benefits the production of ·O. Furthermore, the Ce/Ce redox couple that exists in CeO may act as a trap to capture the electron, which inhibits the recombination of electrons and holes, and further reacts with the adsorbed oxygen on the catalyst surface to generate·O. This approach of introducing Ce/Ce redox pairs as defect centrals to improve superoxide radicals offers an avenue for modifying photocatalytic materials and provides an alternative way to traditional antibiotic strategies in antibacterial applications.
采用水热法合成了具有不同CeO含量的CeO/CuMoO II型异质结。与单独的CuMoO相比，这些异质结催化剂在可见光照射下对两种细菌均表现出优异的抗菌活性。对光催化过程中细菌形态、细菌细胞内含量浓度和活性氧（ROS）的评估表明，ROS氧化细菌细胞膜中的磷脂，导致细胞结构受损和细胞内容物泄漏，最终导致细菌失活。电子顺磁共振（EPR）和ROS清除实验均证实超氧自由基（·O）和e在光催化抗菌活性中起重要作用。将CeO引入CuMoO中可提高异质结的光电性能，促进光生载流子的有效分离，这有利于·O的产生。此外，CeO中存在的Ce/Ce氧化还原对可能作为捕获电子的陷阱，抑制电子和空穴的复合，并进一步与催化剂表面吸附的氧反应生成·O。这种将Ce/Ce氧化还原对作为缺陷中心引入以改善超氧自由基的方法为修饰光催化材料提供了一条途径，并在抗菌应用中为传统抗生素策略提供了一种替代方法。
