Zhang Caixia, Jiang Kai, Liu Qing, Xu Hualing, Ning Kai, Yu Hai Tao, Zhang Maolin, Zhu Jingwen, Chen Min
College of Life Sciences, Shandong Normal University, Ji'nan, 250014, Shandong, China.
National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Agricultural High-Tech Industrial Demonstration Area of the Yellow River Delta of Shandong Province, Dongying, 257000, China.
Plant Cell Rep. 2025 Aug 1;44(8):187. doi: 10.1007/s00299-025-03574-y.
Screening of salt-tolerant soybean varieties, identification of key genes and verification of gene function through salt-tolerance assessment, transcriptome profiling and soybean hairy root transformation experiments. Screening and breeding of salt-tolerant soybean varieties is essential to increase the area and yield of soybean on saline-alkaline soils. In this study, 81 soybean varieties were systematically assessed for salt tolerance during both germination and early seedling development stages. Based on their salt-tolerance capacity, the varieties were categorized into four distinct groups. Physiological analysis revealed that the highly salt-tolerant genotype effectively restricted ions accumulation in roots through compartmentalization mechanisms, while subsequent biochemical assays demonstrated its superior antioxidant enzyme activity (particularly SOD and CAT), thereby mitigating membrane system damage under NaCl stress. Comparative transcriptome profiling between salt-tolerant and sensitive cultivars identified 3588 differentially expressed genes (DEGs) predominantly involved in ion transport, oxidative stress, and photosynthesis. Functional validation through preliminary experiments using the soybean hairy root transformation method highlighted the potential regulatory roles of the candidate gene (Gm10G262850v4) in salt stress responses. These findings provide insights into the mechanisms of soybean salt tolerance and facilitate the breeding of salt-tolerant soybean varieties.
通过耐盐性评估、转录组分析和大豆毛状根转化实验筛选耐盐大豆品种、鉴定关键基因并验证基因功能。筛选和培育耐盐大豆品种对于增加盐碱地大豆种植面积和产量至关重要。本研究对81个大豆品种在萌发期和幼苗早期发育阶段的耐盐性进行了系统评估。根据其耐盐能力，将这些品种分为四个不同的组。生理分析表明，高耐盐基因型通过区室化机制有效限制根中离子积累，随后的生化分析表明其具有优异的抗氧化酶活性（特别是超氧化物歧化酶和过氧化氢酶），从而减轻NaCl胁迫下的膜系统损伤。耐盐和敏感品种之间的比较转录组分析鉴定出3588个差异表达基因（DEG），主要涉及离子运输、氧化应激和光合作用。通过大豆毛状根转化方法进行的初步实验进行功能验证，突出了候选基因（Gm10G262850v4）在盐胁迫反应中的潜在调控作用。这些发现为大豆耐盐机制提供了见解，并促进了耐盐大豆品种的培育。
