Zhang Minghui, Cao Junfeng, Zhang Tianxu, Xu Tao, Yang Liyuan, Li Xiaoyuan, Ji Fengdan, Gao Yingxue, Ali Shahid, Zhang Qingzhu, Zhu Jianhua, Xie Linan
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Front Plant Sci. 2022 May 16;13:870695. doi: 10.3389/fpls.2022.870695. eCollection 2022.
Soybean () is a staple crop and a major source of vegetable protein and vegetable oil. The growth of soybean is dramatically inhibited by salt stress, especially by the excessive toxic Na. Salt Overly Sensitive 1 (SOS1) is the only extensively characterized Na efflux transporter in multiple plant species so far. However, the role of GmSOS1 in soybean salt stress responses remains unclear. Herein, we created three mutants using the CRISPR-Cas9 system in soybean. We found a significant accumulation of Na in the roots of the mutants, resulting in the imbalance of Na and K, which links to impaired Na efflux and increased K efflux in the roots of the mutants under salt stress. Compared to the wild type, our RNA-seq analysis revealed that the roots of the showed preferential up and downregulation of ion transporters under salt stress, supporting impaired stress detection or an inability to develop a comprehensive response to salinity in the mutants. Our findings indicate that the plasma membrane Na/H exchanger GmSOS1 plays a critical role in soybean salt tolerance by maintaining Na homeostasis and provides evidence for molecular breeding to improve salt tolerance in soybean and other crops.
大豆（）是一种主食作物，也是植物蛋白和植物油的主要来源。盐胁迫，尤其是过量的有毒钠，会显著抑制大豆的生长。盐超敏感1（SOS1）是迄今为止在多种植物物种中唯一被广泛表征的钠外排转运蛋白。然而，GmSOS1在大豆盐胁迫反应中的作用仍不清楚。在此，我们利用CRISPR-Cas9系统在大豆中创建了三个突变体。我们发现突变体根中钠大量积累，导致钠钾失衡，这与盐胁迫下突变体根中钠外排受损和钾外排增加有关。与野生型相比，我们的RNA测序分析表明，突变体的根在盐胁迫下离子转运蛋白出现优先上调和下调，这支持了突变体中胁迫检测受损或无法对盐度产生全面反应。我们的研究结果表明，质膜钠/氢交换器GmSOS1通过维持钠稳态在大豆耐盐性中起关键作用，并为通过分子育种提高大豆和其他作物的耐盐性提供了证据。
