Hibino T, Hirasawa Y, Arai M
Cancer Lett. 1985 Dec;29(3):255-63. doi: 10.1016/0304-3835(85)90135-1.
The effects of long-term administration of sodium saccharin on the urinary bladder and stomach of F344 rats were investigated. Sixty-eight male F344 rats, aged 7 weeks at the beginning of the experiment, were maintained on diet supplemented with 5% sodium saccharin for 112 weeks. Animals were killed periodically and investigated for gross and microscopic lesions in the urinary bladder and stomach. Papillary or nodular hyperplasia was evident in the urinary bladder epithelium from 8 weeks onwards although no papillomas or transitional cell carcinomas developed. Lesions observed in the bladders of control animals fed the basal diet without the saccharin supplement were limited throughout the experiment to a few areas of simple hyperplasia. While no changes were apparent in the stomach of control animals, a 100% incidence of hyperkeratosis at the limiting ridge of the forestomach was observed after 80 weeks administration of saccharin, 5 of 20 animals also having papillomas. Furthermore, erosion was pronounced in the glandular stomach of saccharin-treated animals with 4 cases of atypical gland being observed. No histopathological lesions were apparent in the liver, kidneys or spleen of either control or experimental groups.
研究了长期给予糖精钠对F344大鼠膀胱和胃的影响。68只雄性F344大鼠在实验开始时7周龄，在含5%糖精钠的饲料喂养下维持112周。定期处死动物，检查膀胱和胃的大体及微观病变。从8周起，膀胱上皮出现乳头状或结节状增生，尽管未发生乳头状瘤或移行细胞癌。在喂食不含糖精补充剂的基础饲料的对照动物膀胱中观察到的病变在整个实验过程中仅限于少数几个单纯增生区域。对照动物的胃没有明显变化，但在给予糖精80周后，在前胃的界限嵴处观察到100%的角化过度发生率，20只动物中有5只也有乳头状瘤。此外，糖精处理动物的腺胃有明显糜烂，观察到4例非典型腺体。对照组和实验组的肝脏、肾脏或脾脏均未出现组织病理学病变。
