Gabriel L, Miglietta A, Dianzani M U
Chem Biol Interact. 1985 Dec 31;56(2-3):201-12. doi: 10.1016/0009-2797(85)90006-7.
4-Hydroxy-alkenals effect on microtubular system has been investigated, comparing the activity of both biogenic and non-biogenic aldehydes. As these aldehydes react essentially with sulphydryl groups, their action on titratable thiol groups microtubular protein was studied. These compounds evidenced an inhibitory effect on this parameter and on tubulin polymerization, where sulphydryls are essential. 4-Hydroxy-alkenals inhibit tubulin polymerization in a dose-dependent manner (0.1-1 mM), with the exception of 4-hydroxy-octenal, that shows an inhibitory action only in concentrations from 0.5 mM up. Its behaviour is very anomalous: in fact the tubulin-colchicine binding, is stimulated rather than inhibited, whereas such binding is inhibited by the other tested aldehydes. Our present results give then a support for an interaction between 4-hydroxy-alkenals and microtubular protein.
研究了4-羟基烯醛对微管系统的影响，并比较了生物源和非生物源醛的活性。由于这些醛主要与巯基反应，因此研究了它们对微管蛋白可滴定巯基的作用。这些化合物对该参数和微管蛋白聚合表现出抑制作用，而巯基在微管蛋白聚合中至关重要。4-羟基烯醛以剂量依赖性方式（0.1-1 mM）抑制微管蛋白聚合，但4-羟基辛烯醛除外，它仅在浓度高于0.5 mM时显示抑制作用。其行为非常异常：实际上，微管蛋白-秋水仙碱结合受到刺激而非抑制，而其他测试醛则抑制这种结合。因此，我们目前的结果支持了4-羟基烯醛与微管蛋白之间的相互作用。
