Miglietta A, Gabriel L, Gadoni E
Cell Biochem Funct. 1987 Jul;5(3):189-94. doi: 10.1002/cbf.290050306.
Pentanal and hexanal are some of the aldehydes produced by lipid peroxidation, that causes damage to several subcellular structures. Lipoperoxidation products may directly attack cytoskeleton structures, the integrity of which is required for secretion mechanisms, e.g. 4-hydroxy-alkenals after microtubular integrity and function. Purified microtubular protein incubated with pentanal and hexanal at different concentrations revealed a tubulin-aldehyde interaction affecting the polymerization reaction and the colchicine-binding activity. These reactions apparently do not involve sulphydryl groups, and the addition of mercaptoethanol does not protect microtubules from the action of aldehydes, the effect of which is however more homogeneous, as only small differences can be noticed among the various aldehyde concentrations used.
戊醛和己醛是脂质过氧化产生的一些醛类物质，脂质过氧化会对多个亚细胞结构造成损害。脂质过氧化产物可能直接攻击细胞骨架结构，而细胞骨架结构的完整性对于分泌机制是必需的，例如微管完整性和功能受到影响后的4-羟基烯醛。用不同浓度的戊醛和己醛孵育纯化的微管蛋白，结果显示微管蛋白与醛之间的相互作用会影响聚合反应和秋水仙碱结合活性。这些反应显然不涉及巯基，添加巯基乙醇并不能保护微管免受醛的作用，不过醛的作用更为均匀，因为在所使用的各种醛浓度之间只能注意到很小的差异。
