Olivero A, Miglietta A, Gadoni E, Gabriel L
Dipartimento di Medicina ed Oncologia Sperimentale, Sezione di Patologia Generale, Torino, Italy.
Cell Biochem Funct. 1992 Mar;10(1):19-26. doi: 10.1002/cbf.290100104.
The molecular structure of aldehydes is closely related to their antimicrotubular effect. Morphological modifications of the microtubular system in living cells after incubation with certain aldehydes are consistent with biochemical alterations detected in previous research. The microtubular arrangement was visualized by an immunofluorescence technique with antitubulin antibodies, while the content of tubulin in the cells was evaluated by a colchicine binding assay. 2-Nonenal behaved similarly to 4-hydroxynonenal, a lipid peroxidation product, disorganizing microtubular network in 3T3 fibroblasts and decreasing the amounts of tubulin able to bind labelled colchicine. Nonanal did not significantly impair the tubulin characteristics in the cells, despite the fact that it has been shown to be active on the purified microtubular system; benzaldehyde was ineffective. This would appear to explain the mechanisms of interaction of aliphatic aldehydes which might be suitable for use as antimicrotubular drugs.
醛类的分子结构与其抗微管作用密切相关。用某些醛类孵育后，活细胞中微管系统的形态学改变与先前研究中检测到的生化变化一致。通过用抗微管蛋白抗体的免疫荧光技术观察微管排列，同时通过秋水仙碱结合试验评估细胞中微管蛋白的含量。2-壬烯醛的表现与脂质过氧化产物4-羟基壬烯醛相似，它破坏3T3成纤维细胞中的微管网络，并减少能够结合标记秋水仙碱的微管蛋白量。壬醛虽已证明对纯化的微管系统有活性，但并未显著损害细胞中的微管蛋白特性；苯甲醛则无作用。这似乎可以解释脂肪醛作为抗微管药物可能的相互作用机制。
