Zhang Xiaona, Gu Lingdi, Yang Guimin, Liu Can, Huang Kaifeng, Chen Qingfu
Research Center of Buckwheat Industry Technology, College of Life Science, Guizhou Normal University, Guiyang, Guizhou, China.
School of International Education, Guizhou Normal University, Guiyang, Guizhou, China.
Virulence. 2025 Dec;16(1):2543062. doi: 10.1080/21505594.2025.2543062. Epub 2025 Aug 15.
Endophytic colonization of entomopathogenic fungi has garnered significant attention for its role in promoting plant growth. Specifically, has shown a positive effect on the germination of (buckwheat) seeds, but the mechanisms underlying this promotion remain unclear. This study aims to elucidate the colonization process of in seeds during germination stages, quantify the colonization efficiency and tissue specificity of the fungus, and investigate the temporal dynamics of antioxidant enzyme activities and malondialdehyde content triggered by fungal colonization. Furthermore, we evaluated the potential of -colonized seedlings to suppress populations through oviposition inhibition. The results demonstrated for the first time that could successfully colonize germinating seeds during the seed imbibition and germination stages, either by forming dissolution zones with its spores or by germinating and forming mycelia. Initial colonization of all tissues was observed within 16 h, with colonization rates peaking after 5 d, with a preferential colonization rate observed as endosperm > embryo > seed coat. Furthermore, the colonization by enhanced peroxidase (POD) activity in the embryo and reduced malondialdehyde (MDA) content. Seedlings grown after colonization were also found to effectively reduce the number of eggs laid by . These findings provide both theoretical insights and practical foundations for developing a symbiotic system between and seeds.
昆虫病原真菌的内生定殖因其在促进植物生长中的作用而备受关注。具体而言，其已显示出对苦荞种子萌发有积极影响，但这种促进作用背后的机制仍不清楚。本研究旨在阐明苦荞种子在萌发阶段被该真菌定殖的过程，量化真菌的定殖效率和组织特异性，并研究真菌定殖引发的抗氧化酶活性和丙二醛含量的时间动态。此外，我们评估了被该真菌定殖的幼苗通过产卵抑制来抑制小菜蛾种群的潜力。结果首次表明，该真菌能够在种子吸胀和萌发阶段通过其孢子形成溶解区或通过萌发并形成菌丝体成功定殖萌发的苦荞种子。在16小时内观察到所有组织的初始定殖，定殖率在5天后达到峰值，观察到的优先定殖率为胚乳>胚>种皮。此外，该真菌的定殖增强了胚中的过氧化物酶（POD）活性并降低了丙二醛（MDA）含量。还发现定殖后生长的幼苗能有效减少小菜蛾产的卵数。这些发现为开发苦荞与该真菌之间的共生系统提供了理论见解和实践基础。
