Yang Meng, Zhang Chong, Lu Bo-Yun, Zhu Yu-Cheng, Fu Xin-Rui, Wu Yang, Zheng Zi-Tian, Liu Chang-Shuo, Cheng Jin, Huang Hong-Jie, Wang Jian-Quan
Department of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, Beijing, 100191, China.
Institute of Sports Medicine of Peking University, Beijing, 100191, China.
Mater Today Bio. 2025 Jul 23;34:102129. doi: 10.1016/j.mtbio.2025.102129. eCollection 2025 Oct.
Tendon injury is a common condition with potential for disability. Severe injuries such as tendon rupture often require surgical suturing to restore their structure and function. The healing process of tendons is a critical factor affecting clinical outcomes; however, their poor blood supply and low cellular density limit self-regeneration capabilities. This leads to insufficient quality and biomechanical properties of the repaired tendon, playing a significant role in the high rate of re-rupture in clinical practice. Tendon healing involves endogenous (tenocyte proliferation) and exogenous (fibroblast invasion) mechanisms. An imbalance between these two mechanisms often leads to postoperative adhesions, which significantly impacts clinical efficacy. Thus, this project aims to design an injectable, sustained-release methacryloyl gelatin (GelMA) hydrogel microsphere modified with Platelet-Derived Growth Factor-BB (PDGF-BB) and fibronectin (Fn), in conjunction with an anti-adhesion hyaluronic acid methacrylate (HAMA) hydrogel membrane. This combination is designed to promote tendon healing and prevent adhesions. This study will utilize an Achilles tendon rupture animal model to assess the effectiveness of composite microspheres/hydrogel membranes in enhancing tendon repair and preventing adhesions, offering insights for new clinical strategies in tendon rupture treatment.
肌腱损伤是一种常见疾病，具有导致残疾的可能性。严重的肌腱损伤，如肌腱断裂，通常需要手术缝合以恢复其结构和功能。肌腱的愈合过程是影响临床结果的关键因素；然而，其血液供应差和细胞密度低限制了自我再生能力。这导致修复后的肌腱质量和生物力学性能不足，在临床实践中再断裂率高方面起重要作用。肌腱愈合涉及内源性（腱细胞增殖）和外源性（成纤维细胞侵入）机制。这两种机制之间的失衡常导致术后粘连，显著影响临床疗效。因此，本项目旨在设计一种用血小板衍生生长因子 - BB（PDGF - BB）和纤连蛋白（Fn）修饰的可注射、缓释甲基丙烯酰化明胶（GelMA）水凝胶微球，并结合抗粘连甲基丙烯酸透明质酸（HAMA）水凝胶膜。这种组合旨在促进肌腱愈合并防止粘连。本研究将利用跟腱断裂动物模型评估复合微球/水凝胶膜在增强肌腱修复和防止粘连方面的有效性，为肌腱断裂治疗的新临床策略提供见解。
