Chen Jie, Lin Tao, Yuan Yue, Wu Peilin, Dai Shishi, Xu Huan, Zhang Jingyi, Ma Jing
Sichuan Institute for Drug Control (Sichuan Testing Center of Medical Devices), NMPA Key Laboratory for Quality Control and Evaluation of Vaccines and Biological Products, SCMPA Key Laboratory for Quality Monitoring and Risk Assessment of Biological Products, Chengdu 611731, Sichuan, China.
State Key Laboratory of Oral Diseases &National Clinical Research Center for Oral Diseases & Engineering Research Center of Oral Translational Medicine, Ministry of Education & National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
J Stem Cells Regen Med. 2025 Apr 24;21(1):11-18. doi: 10.46582/jsrm.2101003. eCollection 2025.
The significant immunomodulatory capacity of mesenchymal stem cells (MSCs) is increasingly being recognized, making them valuable for the treatment of autoimmune disorders. MSCs influence immune cell behavior during therapy through intercellular communication mediated by extracellular vesicles (EVs). Moreover, MSC-derived apoptotic vesicles (apoEVs) can also exert immunomodulatory functions. This study compared the effects of dental follicle stem cells (DFSCs) and smaller apoptotic vesicles (apoSEVs) derived from DFSCs on the proliferation of peripheral blood mononuclear cells (PBMC), as well as their impact on T cell subpopulations and inflammatory factor expression. The results showed that apoSEVs derived from DFSCs significantly enhanced PBMC proliferation, inhibited Th1, Th17, and Treg cell populations, and reduced IFN-γ and TNF-α expression levels. These findings demonstrate that apoSEVsapoSEVs from DFSCs can effectively regulate immune responses in a manner similar to that of DFSCs themselves.
间充质干细胞（MSCs）显著的免疫调节能力日益受到认可，这使其在自身免疫性疾病的治疗中具有重要价值。MSCs在治疗过程中通过细胞外囊泡（EVs）介导的细胞间通讯影响免疫细胞行为。此外，源自MSCs的凋亡囊泡（apoEVs）也可发挥免疫调节功能。本研究比较了牙囊干细胞（DFSCs）及其衍生的较小凋亡囊泡（apoSEVs）对外周血单个核细胞（PBMC）增殖的影响，以及它们对T细胞亚群和炎症因子表达的影响。结果表明，源自DFSCs的apoSEVs显著增强PBMC增殖，抑制Th1、Th17和Treg细胞群体，并降低IFN-γ和TNF-α表达水平。这些发现表明，源自DFSCs的apoSEVs能够以类似于DFSCs自身的方式有效调节免疫反应。
