Baransel Gizem Şafak, Yücel Oğuz, Yıldırım Eren, Kalyon Göksenin, Emik Serkan, Erol Ayşe, Kara Neslihan Turgut
Program of Molecular Biotechnology and Genetics, Institute of Science, Istanbul University, 34116 Istanbul, Turkey.
Department of Chemical Engineering, Faculty of Engineering, Istanbul University-Cerrahpaşa, 34320 Avcılar, Istanbul, Turkey.
Physiol Mol Biol Plants. 2025 Jun;31(6):959-978. doi: 10.1007/s12298-025-01616-z. Epub 2025 Jun 28.
The study investigated the effects and potential applications of green-synthesized silver nanoparticles (AgNPs) on cotton plants under in vitro drought stress. AgNPs were synthesized using cotton seed oil cake extract (CSOCE) as a stabilizing and reducing agent. The secondary metabolite content of CSOCE was analyzed using High Performance Liquid Chromatography (HPLC). Characterization of synthesized AgNPs was performed using Dynamic Light Scattering (DLS), polydispersity index (PDI), Zeta Potential (ZP), Scanning Electron Microscopy and Energy Dispersive X-Ray Spectroscopy (SEM-EDS), X-Ray Diffraction Analysis (XRD), Ultraviolet-Visible Light Spectroscopy (UV-Vis spectroscopy), and Fourier Transform Infrared Spectrometry (FTIR) analyses. According to SEM, the nanoparticle sizes varied between 50 and 100 nm. ZP was - 28.7 mV and PDI value was 0.65 according to DLS results. The experimental groups were: (1) MS medium (control group), (2) PEG, (3) AgNP, and (4) PEG + AgNP. Plants were transferred to the respective media, cultured for three days, and subsequently analyzed. Morphological parameters including root number, root and shoot lengths, and leaf surface area were measured, while physiological traits such as relative water content, biomass accumulation, osmolyte accumulation, and photosynthetic pigment contents were assessed. Molecular analyses were conducted to examine the relative gene expression of drought stress-associated genes, including , , , , , , and . The results demonstrated that the application of AgNPs alleviated the adverse effects of in vitro drought stress on plants. These findings suggest that green synthesized AgNPs hold significant potential as agents to mitigate drought stress in plants.
The online version contains supplementary material available at 10.1007/s12298-025-01616-z.
本研究调查了绿色合成银纳米颗粒（AgNPs）在体外干旱胁迫下对棉花植株的影响及潜在应用。使用棉籽油饼提取物（CSOCE）作为稳定剂和还原剂合成了AgNPs。采用高效液相色谱（HPLC）分析CSOCE的次生代谢产物含量。使用动态光散射（DLS）、多分散指数（PDI）、zeta电位（ZP）、扫描电子显微镜和能量色散X射线光谱（SEM-EDS）、X射线衍射分析（XRD）、紫外-可见光谱（UV-Vis光谱）和傅里叶变换红外光谱（FTIR）分析对合成的AgNPs进行表征。根据扫描电子显微镜（SEM），纳米颗粒尺寸在50至100纳米之间变化。根据动态光散射（DLS）结果，zeta电位为-28.7毫伏，多分散指数（PDI）值为0.65。实验组为：（1）MS培养基（对照组），（2）聚乙二醇（PEG），（3）AgNP，和（4）PEG + AgNP。将植株转移到各自的培养基中，培养三天，随后进行分析。测量了包括根数、根长和茎长以及叶表面积在内的形态学参数，同时评估了诸如相对含水量、生物量积累、渗透溶质积累和光合色素含量等生理特性。进行分子分析以检测干旱胁迫相关基因的相对基因表达，包括 、 、 、 、 、 和 。结果表明，AgNPs的应用减轻了体外干旱胁迫对 植株的不利影响。这些发现表明，绿色合成的AgNPs作为减轻植物干旱胁迫的试剂具有巨大潜力。
在线版本包含可在10.1007/s12298-025-01616-z获取的补充材料。
