Silva Fabiane N, Sampaio Igor C F, Chaves João Marcos de S, Sena Luise de O, Emmerich Iana T, de Jesus Gabriel Lucas S, Costa Floriatan S, Mendes Adriano A, Dos Santos Jailma B, de Moura Isabela V L, Tavares Iasnaia Maria de C, Irfan Muhammad, Franco Marcelo
Department of Exact Sciences and Natural, State University of Southwest Bahia, Itapetinga 45700-000, Brazil.
Biotransformation and Organic Biocatalysis Research Group, Department of Exact Sciences, Santa Cruz State University, Ilhéus 45654-370, Brazil.
ACS Omega. 2025 Jul 16;10(29):32161-32173. doi: 10.1021/acsomega.5c03929. eCollection 2025 Jul 29.
Cellulases play a crucial role in cellulose degradation and lignocellulosic biomass conversion. The cost-effective production of these enzymes can be achieved using in solid-state fermentation with low-cost substrates. In this study, the optimal composition of the substrate mixture (80% cocoa bean shell and 20% forage palm) was determined by using the Simplex-Centroid design. The optimal fermentation conditions (28 °C, 60% moisture for 96 h) were established through the Doehlert design. Enzymatic characterization revealed activity over a broad pH range (5-9) for up to 5 h, a thermal stability of 70% at 70 °C, and halophilic properties, with a 278% increase in activity in the presence of NaCl. Additionally, the enzymes were activated by Ca (+60%) and DMSO (+69%). The carbohydrase blend (CB) effectively hydrolyzed rice husk, generating 13 mg of reducing sugars per gram of biomass. HPLC analysis identified glucose (7.24 mg/L) and fructose (11.21 mg/L), confirming the synergistic activity of the endoglucanase and isomerase enzymes. These results underscore the innovative and biotechnologically relevant nature of the enzymatic formulation, particularly in the sustainable production of fructose-rich syrup from rice husk. The approach holds significant promise for applications in bioenergy generation and the valorization of food waste.
纤维素酶在纤维素降解和木质纤维素生物质转化中起着关键作用。使用低成本底物进行固态发酵可以实现这些酶的经济高效生产。在本研究中，采用单纯形重心设计确定了底物混合物的最佳组成（80%可可豆壳和20%饲料棕榈）。通过Doehlert设计确定了最佳发酵条件（28°C、60%湿度，持续96小时）。酶学特性表明，该酶在较宽的pH范围（5-9）内可保持5小时的活性，在70°C时热稳定性为70%，具有嗜盐特性，在NaCl存在下活性增加278%。此外，该酶被Ca（+60%）和DMSO（+69%）激活。碳水化合物酶混合物（CB）有效地水解了稻壳，每克生物质产生13毫克还原糖。HPLC分析鉴定出葡萄糖（7.24毫克/升）和果糖（11.21毫克/升），证实了内切葡聚糖酶和异构酶的协同活性。这些结果强调了酶制剂的创新性和生物技术相关性，特别是在从稻壳可持续生产富含果糖的糖浆方面。该方法在生物能源生产和食物垃圾增值利用方面具有巨大的应用前景。
