Arcangeli Danilo, Mariani Federica, Gualandi Isacco, Ragnucci Manuel, Decataldo Francesco, Bonafè Filippo, Tonelli Domenica, Fraboni Beatrice, Scavetta Erika
Organic Bioelectronics Laboratory, Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Department of Industrial Chemistry "Toso Montanari", University of Bologna, Via Piero Gobetti 85, Bologna 40129, Italy.
ACS Omega. 2025 Jul 18;10(29):32202-32215. doi: 10.1021/acsomega.5c03720. eCollection 2025 Jul 29.
Excreted biological fluids represent a matrix rich in useful biomarkers that can be correlated with a vast number of medical conditions and physiological processes, for both diagnostic and therapeutic purposes. Among the plethora of medically relevant analytes, the calcium ion is a target of prime interest, as it is present in many biofluids such as saliva, sweat, and wound exudate. Its concentration can be associated with a variety of afflictions, such as osteoporosis, periodontal diseases, kidney stone formation, parathyroid gland activity, and impaired wound healing. In this work, we present a novel device architecture for the potentiostatic, reference electrode-free detection of calcium, renovating the original organic electrochemical transistor (OECT) model by Wrighton. The sensing event takes place on the gate, functionalized with a Ca-selective membrane, whose potentiometric shift is used to modulate the current flowing through a poly-(3,4-ethylenedioxythiophene) perchlorate (PEDOT:ClO) interdigitated channel. Calcium sensing was performed in buffer solutions, reporting a linear range between 0.002 and 19 mM. The sensor proved to cover the biologically relevant range for calcium ion concentrations in complex media, using synthetic wound exudate as a model biomatrix, and it reported excellent selectivity under exposure to high concentrations of interfering species.
排泄的生物流体是一种富含有用生物标志物的基质，可用于诊断和治疗目的，与大量医学病症和生理过程相关。在众多与医学相关的分析物中，钙离子是主要关注的目标，因为它存在于许多生物流体中，如唾液、汗液和伤口渗出液。其浓度可能与多种疾病有关，如骨质疏松症、牙周疾病、肾结石形成、甲状旁腺活动以及伤口愈合受损。在这项工作中，我们提出了一种用于恒电位、无参比电极检测钙的新型器件架构，对Wrighton最初的有机电化学晶体管（OECT）模型进行了改进。传感事件发生在栅极上，该栅极用钙选择性膜进行功能化，其电位变化用于调制流过聚（3,4-乙撑二氧噻吩）高氯酸盐（PEDOT:ClO）叉指通道的电流。在缓冲溶液中进行了钙传感，报告的线性范围为0.002至19 mM。该传感器以合成伤口渗出液作为模型生物基质，证明能够覆盖复杂介质中钙离子浓度的生物学相关范围，并且在暴露于高浓度干扰物质时表现出优异的选择性。
