Yoon Jung Pyo, Kim Tae Hun, Park So Yoon, Yoon Ki Young, Kim Hyung Min
EMBIOME Co. Ltd., 260 Changnyong-daero, Yeongtong-gu, Suwon 16229, Republic of Korea.
Int J Syst Evol Microbiol. 2025 Aug;75(8). doi: 10.1099/ijsem.0.006884.
Two novel pink-pigmented bacteria, designated strains MA0201 and ID0610, were isolated from a car air conditioning system. The isolates were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and facultatively methylotrophic. Cells of strains MA0201 and ID0610 are motile with polar flagella (lophotrichous) and a polar flagellum (monotrichous), respectively. Both strains indicated catalase- and oxidase-positive activities. The growth of strain MA0201 was observed at 15-35 °C, at pH 5.0-7.5 and in the presence of 0-0.5% (w/v) NaCl, while the growth of strain ID0610 indicated at 20-40 °C, at pH 5.0-7.5 and in the presence of 0-0.5% (w/v) NaCl. Ubiquinone-10 was the major respiratory quinone in both strains. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were identified as the major polar lipids in both strains, and an unidentified lipid and an unidentified aminolipid were additionally identified in strains MA0201 and ID0610, respectively. The major cellular fatty acids (>5%) were summed feature 8 (C c and/or C c) and C cyclo 8 in strain MA0201, while strain ID0610 contained summed feature 8 (C c and/or C c) and C. Phylogenetic and phylogenomic analyses showed that strains MA0201 and ID0610 formed distinct phylogenic lineages within the genus . Strain MA0201 exhibited high 16S rRNA gene sequence similarity to PMB02 (98.8%), and digital DNA-DNA hybridization (dDDH) and orthologous average nt identity (ANI) values were 38.8% and 90.0%, respectively. Strain ID0610 showed high 16S rRNA gene sequence similarity to AR24 (98.5%), and dDDH and ANI values were 27.2% and 83.4%, respectively, indicating that both strains represent novel species of the genus . Based on their phenotypic, chemotaxonomic and molecular properties, strains MA0201 and ID0610 are proposed to represent two novel species within the genus , namely, sp. nov. (type strain MA0201=KACC 23846=JCM 37404) and sp. nov. (type strain ID0610=KACC 23847=JCM 37403).
从汽车空调系统中分离出两株新型粉红色色素细菌，分别命名为MA0201和ID0610菌株。分离菌株为革兰氏阴性、需氧、无芽孢、杆状且兼性甲基营养型。MA0201和ID0610菌株的细胞分别通过极生鞭毛（束生鞭毛）和极生鞭毛（单生鞭毛）运动。两株菌均显示过氧化氢酶和氧化酶阳性活性。MA0201菌株在15 - 35°C、pH 5.0 - 7.5以及0 - 0.5%（w/v）NaCl存在的条件下生长，而ID0610菌株在20 - 40°C、pH 5.0 - 7.5以及0 - 0.5%（w/v）NaCl存在的条件下生长。泛醌 - 10是两株菌中的主要呼吸醌。磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油被鉴定为两株菌中的主要极性脂质，并且在MA0201菌株中额外鉴定出一种未鉴定的脂质，在ID0610菌株中额外鉴定出一种未鉴定的氨基脂质。MA0201菌株中主要的细胞脂肪酸（>5%）是总和特征8（C c和/或C c）和C环8，而ID0610菌株包含总和特征8（C c和/或C c）和C。系统发育和系统基因组分析表明，MA0201和ID0610菌株在该属内形成了不同的系统发育谱系。MA0201菌株与PMB02的16S rRNA基因序列相似度较高（98.8%），数字DNA - DNA杂交（dDDH）和直系同源平均核苷酸同一性（ANI）值分别为38.8%和90.0%。ID0610菌株与AR24的16S rRNA基因序列相似度较高（98.5%），dDDH和ANI值分别为27.2%和83.4%，这表明两株菌均代表该属的新物种。基于它们的表型、化学分类和分子特性，提议MA0201和ID0610菌株代表该属内的两个新物种，即sp. nov.（模式菌株MA0201 = KACC 23846 = JCM 37404）和sp. nov.（模式菌株ID0610 = KACC 23847 = JCM 37403）。
