Nammali Achararak, Duangupama Thitikorn, Pittayakhajonwut Pattama, Intaraudom Chakapong, Suriyachadkun Chanwit, He Ya-Wen, Tanasupawat Somboon, Thawai Chitti
Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phaholyothin Road, Klong Luang, Pathum Thani 12120, Thailand.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005626.
An actinobacterium strain PLK6-54 was isolated from Lankwai peat swamp forest soil collected from Yala province, Thailand. Strain PLK6-54 exhibited morphological and chemotaxonomic properties described for the genus . It formed a spiral spore chain directly on aerial mycelium. Growth was observed between 20 and 40 °C and at pH 5-8. The maximum NaCl for growth was 2 % (w/v). ll-Diaminopimelic acid, arabinose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside were detected as the phospholipids. The major menaquinones were MK-10(H) and MK-9(H). The major cellular fatty acids were iso-C, anteiso-C and iso-C. 16S rRNA gene sequence data supported the assignment of strain PLK6-54 to the genus and showed that NBRC 102073 (99.0 %) was the closest relative. Moreover, the average nucleotide identity-blast (85.5 %) and digital DNA-DNA hybridization (30.7 %) values reported between strain PLK6-54 and its closest neighbour were below the threshold values for delineation of a novel species. Strain PLK6-54 could be distinguished from related validly described species by several phenotypic properties. The combination of genotypic and phenotypic data indicated that strain PLK6-54 is representative of a novel species of the genus . The name sp. nov. is proposed for strain PLK6-54. The type strain is PLK6-54 (=TBRC 11250=NBRC 114297).
从泰国也拉府采集的兰卡威泥炭沼泽森林土壤中分离出一株放线菌菌株PLK6 - 54。菌株PLK6 - 54表现出该属所描述的形态和化学分类学特征。它直接在气生菌丝体上形成螺旋状孢子链。在20至40°C以及pH值5 - 8的条件下观察到其生长。生长的最大NaCl浓度为2%（w/v）。在全细胞水解产物中检测到ll - 二氨基庚二酸、阿拉伯糖和核糖。检测到双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷作为磷脂。主要的甲基萘醌是MK - 10(H)和MK - 9(H)。主要的细胞脂肪酸是异-C、anteiso - C和异-C。16S rRNA基因序列数据支持将菌株PLK6 - 54归入该属，并表明NBRC 102073（99.0%）是其最接近的亲缘种。此外，菌株PLK6 - 54与其最接近的亲缘种之间报道的平均核苷酸同一性- blast（85.5%）和数字DNA - DNA杂交（30.7%）值低于新物种界定的阈值。菌株PLK6 - 54可通过几个表型特征与相关的已有效描述的该属物种区分开来。基因型和表型数据的结合表明菌株PLK6 - 54代表了该属的一个新物种。提议将菌株PLK6 - 54命名为新种。模式菌株是PLK6 - 54（=TBRC 11250=NBRC 114297）。
