Cao Yun-Fei, He Dong-Yan, Wang Yu-Ruo, Cao Ke, Li Yang, Gao Jia-Wei, Wang Xing-Cheng, Xu Lin, Sun Cong, Yu Xiao-Yun
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, 310004, People's Republic of China.
Curr Microbiol. 2025 Jul 30;82(9):423. doi: 10.1007/s00284-025-04415-7.
A Gram-stain-negative, rod-shaped bacterial strain, designated GZD-96, characterized by strictly aerobic metabolism, was isolated from tidal flat collected at Sanbei Shoal, Ningbo, China. This strictly aerobic strain formed orange, round, convex colonies on marine agar at 37 °C. It exhibited growth at 10-40 °C (optimum: 37 °C), 0-7% NaCl (optimum: 1%), and pH 5.5-9.5 (optimum: pH 8.0). Microscopic examination revealed short rod-shaped cells measuring 0.4-0.5 μm in width and 1.0-1.1 μm in length. The respiratory quinone profile of strain GZD-96 was dominated by menaquinone-6. Polar lipids comprised phosphatidylethanolamine, additional lipids, identified amino phospholipids, glycolipids, and amino lipids. The predominant fatty acids (> 10%) included Summed feature 3 (comprising C ω7c and/or C ω6c), iso-C, C, and iso-C 3-OH. The genomic DNA of strain GZD-96 exhibited a G + C content of 39.4%, as determined by genome sequence analysis. Phylogenetic analysis based on the 16S rRNA gene sequence indicated the closest relative to be Arenibacter troitsensis KMM 3674, with a sequence similarity of 94.7%. The average nucleotide identity (ANI), digit DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between strain GZD-96 and closely related taxa were 69.79-70.54%, 16.80-18.20%, and 65.57-67.83%, respectively. Based on phylogenetic, chemotaxonomic, and genomic evidence, strain GZD-96 is proposed to represent a novel genus and species within the family Flavobacteriaceae, for which the name Aurantibrevibacter litoralis gen. nov., sp. nov. is proposed. The type strain is GZD-96 (= MCCC 1K08935 = KCTC 102227).
从中国宁波三北浅滩采集的潮滩中分离出一株革兰氏染色阴性、杆状的细菌菌株，命名为GZD-96，其特征为严格需氧代谢。该严格需氧菌株在37℃的海洋琼脂上形成橙色、圆形、凸起的菌落。它在10-40℃（最适温度：37℃）、0-7% NaCl（最适浓度：1%）和pH 5.5-9.5（最适pH：8.0）条件下生长。显微镜检查显示，细胞呈短杆状，宽度为0.4-0.5μm，长度为1.0-1.1μm。菌株GZD-96的呼吸醌谱以甲基萘醌-6为主。极性脂质包括磷脂酰乙醇胺、其他脂质、已鉴定的氨基磷脂、糖脂和氨基脂。主要脂肪酸（>10%）包括总和特征3（由C ω7c和/或C ω6c组成）、异-C、C和异-C 3-OH。通过基因组序列分析确定，菌株GZD-96的基因组DNA的G+C含量为39.4%。基于16S rRNA基因序列的系统发育分析表明，与其亲缘关系最近的是特罗伊茨滨海杆菌Arenibacter troitsensis KMM 3674，序列相似性为94.7%。菌株GZD-96与密切相关分类群之间的平均核苷酸同一性（ANI）、数字DNA-DNA杂交（dDDH）和平均氨基酸同一性（AAI）值分别为69.79-70.54%、16.80-18.20%和65.57-67.83%。基于系统发育、化学分类和基因组证据，菌株GZD-96被提议代表黄杆菌科中的一个新属和新种，为此提出新属名为Aurantibrevibacter litoralis gen. nov.，新种名为Aurantibrevibacter litoralis sp. nov.。模式菌株为GZD-96（=MCCC 1K08935=KCTC 102227）。
