Qiu Le, Xing Danlei, Zhu Yuanqiang, Zhuang Zhiwei, Sun Yexiang, Gong Peng, Chen Xulin
Department of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Front Pharmacol. 2025 Jul 21;16:1511741. doi: 10.3389/fphar.2025.1511741. eCollection 2025.
As a traditional Chinese compound formulation, No.8 burn ointment has demonstrated notable efficacy over half a century of clinical use, particularly in facilitating the healing of burn wounds.
This study integrates clinical data, liquid chromatography-mass spectrometry, network pharmacology and experimental validation to elucidate the mechanisms by which No.8 burn ointment promotes burn wound healing and to identify its key active components.
The findings reveal that the ointment may accelerate wound repair by modulating the HIF-1 signaling pathway. The network pharmacology analysis has identified HIF-1α as a core target of the No.8 burn ointment's action and has identified the key active molecules β-Elemonic acid and Rubiadin, which interact with the HIF-1α protein and influence the HIF-1 pathway.
This research not only provides a theoretical foundation for the selection of targeted drugs in burn treatment and the optimization of traditional Chinese compounds but also provides new ideas for future research in related fields.
作为一种传统的中药复方制剂，烧伤八号膏在半个多世纪的临床应用中已显示出显著疗效，尤其是在促进烧伤创面愈合方面。
本研究整合临床数据、液相色谱 - 质谱联用、网络药理学和实验验证，以阐明烧伤八号膏促进烧伤创面愈合的机制，并确定其关键活性成分。
研究结果表明，该药膏可能通过调节缺氧诱导因子 -1（HIF -1）信号通路来加速伤口修复。网络药理学分析已确定HIF -1α为烧伤八号膏作用的核心靶点，并确定了关键活性分子β - 榄香烯酸和茜草素，它们与HIF -1α蛋白相互作用并影响HIF -1通路。
本研究不仅为烧伤治疗中靶向药物的选择和中药复方的优化提供了理论基础，也为相关领域的未来研究提供了新思路。
